產生伏馬鐮孢毒素B（下標 1）之鐮孢菌鑑定與其產毒的影響因子

Identification for Fusarium Species Producing Fumonisin B(subscript 1) and Factors Affecting the Mycotoxin Production

林秀橤(S. R. Lin) ； 鄧資新(T. S. Deng) ； 林宗俊(T. C. Lin) ； 范揚廣(Y. K. Fan) ； 黃振文(J. W. Huang)

《植物病理學會刊》 19卷3期 (2010/09) Pp. 191-200

https://doi.org/10.6649/PPB.201009_19(3).0003

摘要

自水稻與玉米穀粒上分離鐮孢菌屬Liseola群真菌，共獲得16菌株。隨後以Fumonisin kit及HPLC檢測各菌株是否可產生伏馬鐮孢毒素B1 (fumonisin B1, FB1)，結果發現ST-P01（來自玉米飼料）、PR-14、PR-44及PR-143（來自稻穀）等四株菌株均具有產生FB1毒素的能力；其中以ST-P01菌株產生FB1毒素的量最高，達518.52ppm。利用孢子形態、大小、產孢方式及比對核糖體DNA（rDNA）之LSU-D1/D2區域序列等方法，將這些菌株的學名鑑定為Fusarium proliferatum （Matsushima） Nirenberg。進一步，利用玉米液態培養基培養ST-P01菌株，評估培養溫度、日數、培養基質種類等因子對其產生FB1毒素的影響，結果發現靜置培養ST-P01五日後，才可測到FB1毒素的產生，且在32℃的產毒量最高。以小麥、燕麥、裸麥、蕎麥、糙米、紫糯米、白米、小米及玉米等九種液態培養基質分別培養ST-P01菌株，結果發現其在紫糯米粉液態培養基產生FB1毒素的量最高，達35.43ppm，其次在玉米粉液態培養基上的產量則為34.35ppm；至於以裸麥粉液態培養的產毒量最少，僅有2.96ppm。

關鍵字

伏馬鐮孢毒素B（下標 1） ； 鐮孢菌屬 ； Fusarium proliferatum ； 培養因子 ； 真菌毒素

並列摘要

The fumonisin B1 (FB1) production ability of 16 Fusarium isolates in the section Liseola obtained from rice and corn by Nash-PCNB selective medium was examined and evaluated by using Fumonisin kit and HPLC. Four isolates, ST-P01, PR-14, PR-44, and PR-143 were found able to produce FB1 and subsequently identified as Fusarium proliferatum (Matsushima) Nirenberg based on conidial morphology, conidigenous cell, and ribosomal DNA (rDNA) LSU-D1/D2 domain sequencing of these isolates. Among the four isolates, a highest amount of FB1 production, up to 518.52 ppm, was obtained from isolate ST-P01. In advance, the factors affecting FB1 production were studied. The FB1 production by isolate ST-P01 could be detected five days after its static culturing, and the highest production of FB1 was at 32℃ compared to the other temperature treatments. Isolate ST-P01 produced the highest amount of FB1 up to 35.43 ppm in purple glutinous rice meal liquid medium among nine substrates, including purple glutinous rice, unpolished rice, rice, millet, wheat, corn, oat, naked barley, and buckwheat. Isolate ST-P01 cultured in corn meal liquid medium could produce 34.35 ppm of FB1. However, the less one was at 2.96 ppm when isolate ST-P01 was cultured in naked barley meal liquid medium.

並列關鍵字

fumonisin B ； Genus Fusarium ； Fusarium proliferatum ； cultural factors ； mycotoxin