感染天竺葵之胡瓜嵌紋病毒之鑑定與特性分析

Identification and Molecular Characterization of Cucumber mosaic virus Isolated from Pelargonium hortorumin Taiwan

吳沛育(P. Y. Wu) ； 陳玉琪(Y. C. Chen) ； 王惠亮(H. L. Wang)

《植物病理學會刊》 22卷2期 (2013/06) Pp. 105-119

https://doi.org/10.6649/PPB.201306_22(2).0003

摘要

天竺葵（Pelargonium hortorum）為牻牛兒苗科（Geraniaceae）、天竺葵屬（Pelargonium）之多年生宿根花卉，原產於非洲南部。花色多樣，花期極長，適合花壇或盆栽。2010年於彰化田尾發現葉片有明顯葉脈黃化（vein-clearing）及黃斑（chlorotic spots）病徵的天竺葵植株。經三次單斑分離後獲得一純系病毒分離株，稱為GE 1-3。初步經寄主範圍測試、電子顯微鏡觀察、血清學鑑定及利用胡瓜嵌紋病毒（Cucumber mosaic virus，CMV）之專一性引子對（CPr/CPf）進行反轉錄聚合酶連鎖反應（reverse-transcription polymerase chain reaction，RT-PCR），鑑定結果為CMV，暫稱其為CMVGE 1-3。CMV GE 1-3病毒全長RNA 1、RNA 2、RNA 3分別具有3,361、3,047及2,221個核苷酸。將CMV GE 1-3與National Center for Biotechnology Information（NCBI）網站登錄之其他亞群CMV序列進行比對發現：在RNA 3之5'端非轉譯區比較上，顯示與IB亞群相同度最高達98%，在鞘蛋白序列相同度比較上，與IB亞群義大利Tfn系統（Accession No. Y16926）及台灣NT 9系統（Accession No. D28780），核苷酸相同度達95%；胺基酸相同度與IB亞群義大利Tfn系統達100%，與台灣NT9系統達99%。綜上，證實感染天竺葵之CMV GE 1-3分離株為CMV的天竺葵分離株，且為IB亞群。本報告為台灣地區CMV感染天竺葵之首篇紀錄。

關鍵字

天竺葵 ； 病徵 ； 反轉錄聚合酶連鎖反應 ； 胡瓜嵌紋病毒 ； 序列相同度

並列摘要

Geranium (Pelargonium hortorum), a popular bed and potting plant, is a perennial ornamental of the family Geraniaceae oringinated from southern Africa. Geranium plants with chlorotic spots on leaves, which is a typical symptom of of viral infection, were collected at Tianwei, Changhua in 2010.A virus, with a diameter of about 28 nm, was isolated via three successive single lesion isolations on Chenopodium quinoa and was designated as GE 1-3. The preparations of purified GE 1-3 positively reacted with antiserum against Cucumber mosaic virus (CMV) in indirect enzyme-linked immunosorbent assay (indirect ELISA) indicating GE-13 is a CMV isolate. The relative molecular weight of the virus coat protein (CP) was estimated to be about 26.9 kDa by SDS-PAGE and the CP can be detected by the CMV antiserum in western blots. The nucleotide sequences of full-length genome of GE 1-3 have been determined. The genomic RNAs 1, 2 and 3 consist of 3361, 3047, 2221 nucleotides, respectively. Comparison of sequence identities based on the nucleotide and deduced amino acid sequences of individual genes among GE 1-3 and that of other documented CMV isoaltes indicating that the GE 1-3, the virus causing chlorotic spots on geranium leaves, is a CMV isolate of subgroup IB. The results of this study indicate that, this is the very first report of a subgroup ΙB CMV infects geranium plants.

並列關鍵字

geranium ； symptom ； Cucumber mosaic virus (CMV) ； sequence identity