Purification and Characterization of Acidic Protease from Aspergillus oryzae BCRC 30118

The acidic protease was purified from 4-day cultivation of Aspergillus oryzae BCRC 30118 by DEAE Sephacel and Sephacryl S-200 HR chromatographs. The specific activity, yield and purification fold were 117.62 kU/mg, 15.1% and 6.6, respectively. The molecular weight (M) was 41.0 kDa, while the optimal pH and temperature were 3.0 and 60°C, respectively. It was stable at pH 3.0-6.0 and 4-35°C. However, it was inhibited by Fe^2+, Hg^2+, Fe^3+ and pepstatin A, and slightly by leupeptin and TPCK. According to substrate specificity and inhibitor study, it was a cysteine protease with activation energy of 37.5 kcal/mol. Its Km, Vmax, Kcat and Kcat/Km for the hydrolysis of hemoglobin were 0.12 mM, 14.29 μmol/min, 14.55 sec^(-1) and 125.80 (sec^(-1) mM^(-1)), respectively.

並列關鍵字

purification ； acidic protease ； Aspergillus oryzae.

參考文獻

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延伸閱讀

Pong, C. Y., Chou, Y. H., Nieh, F. P., & Jiang, S. T. (2005). Purification and Characterization of Acidic Protease from Aspergillus oryzae. 臺灣水產學會刊, 32(3), 253-264. https://doi.org/10.29822/JFST.200509.0006
謝東諺（2011）。Purification and characterization of a chitinase and a protease from Bacillus cereus TKU028 and their applications〔碩士論文，淡江大學〕。華藝線上圖書館。https://doi.org/10.6846/TKU.2011.00678
Ghazi M. Aziz、Hala M. Ali（2013）。Purification and Characterization of Agarase from Bacillus sp., H12。Current Research Journal of Biological Sciences，5(1)，13-18。https://www.airitilibrary.com/Article/Detail?DocID=20410778-201301-201510300014-201510300014-13-18
Silvestre, M. P., Morais, H. A., Silva, V. D., & Silva, M. R. (2014). Action of Bacillus licheniformis and Aspergillus oryzae Proteases to Obtain Hydrolyzed Proteins with High Antioxidant Activity. Research in Health and Nutrition, 2(), 44-48. https://www.airitilibrary.com/Article/Detail?DocID=P20150529010-201410-201508050022-201508050022-44-48
朱廷輝（2010）。Using ORP response as a parameter to investigateanaerobic fermentation byClostridium acetobutylicum ATCC 824〔碩士論文，朝陽科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0078-2611201410131212

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Purification and Characterization of Acidic Protease from Aspergillus oryzae BCRC 30118

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