Identification and Analysis of White Spot Syndrome Virus (WSSV) Envelope Protein VP41A

Po-Yu Huang ； Pei-Jung Chen ； Huai-Ting Huang ； Li-Li Chen

《Journal of Marine Science and Technology》 21卷特刊 (2013/12) Pp. 234-240

https://doi.org/10.6119/JMST-013-1220-13

並列摘要

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimp in Taiwan and many other countries. WSSV, a kind of large enveloped DNA virus, has a wide host range among crustaceans. During the first stage to infect a target cell, the enveloped virus must combine itself with the receptor on the host cell membrane via an envelope protein. In WSSV, binding of envelope protein with the receptor of the host cell membrane was revealed in a number of previous studies. WSSV envelope proteins were also considered to form a protein complex, at some stage in all research to be named ”infectome”. The WSSV envelope protein VP41A, with the predicted molecular weight of about 41 kDa and having a transmembrane region at its C-terminus, is identified in this study. The interaction of VP41A with the virus' major envelope protein VP26 is revealed by His pull-down and liquid chromatography tandem mass spectrometry. Furthermore, the protein-protein interaction of VP41A with VP28 and VP56 is verified by far western blotting. This data indicate that VP41A participates in the formation of a WSSV protein complex that may be involved in WSSV infection. These findings may identify certain areas for further WSSV research.

並列關鍵字

WSSV ； envelope protein ； infectome ； His pull-down assay ； far western blotting

參考文獻

Chang, Y. S., Liu, W. J., Chou, T. L., Lee, Y. T., and Lee, T. L., “Characterization of white spot syndrome virus envelope protein VP51A and its interaction with viral tegument protein VP26,” Journal of Virology, Vol. 82, No. 22, pp. 12555-12564 (2008).

Chang, Y. S., Liu, W. J., Lee, C. C., Chou, T. L., Lee, Y. T., Wu, T. S., Huang, J. Y., Huang, W. T., Lee, T. L., Kou, G. H., Wang, A. H. J., and Lo, C. F., “A 3D model of the membrane protein complex formed by the white spot syndrome virus structural proteins,” PloS One, Vol. 5, No. 5, e10718 (2010).

Chen, J., Li, Z., and Hew, C. L., “Characterization of a novel envelope protein WSV010 of shrimp white spot syndrome virus and its interaction with a major viral structural protein VP24,” Virology, Vol. 364, No. 1, pp. 208-213 (2007).

Chen, K. Y., Hsu, T. C., Huang, P. Y., Kang, S. T., Lo, C. F., Huang, W. P., and Chen, L. L., “Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection,” Fish and Shellfish Immunology, Vol. 27, No. 3, pp. 460-465 (2009).

Chen, L. L., Lu, L. C., Wu, W. J., Lo, C. F., and Huang, W. P., “White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP),” Diseases of Aquatic Organisms, Vol. 74, No. 3, pp. 171-178 (2007).