Objective: The fatigue and influencing factors in high-tech Industry Method: This study adopts cross-sectional research design to the anonymous questionnaire was used to collect demographics, job characteristics, health characteristics and fatigue data. Results: The subjects were male, aged between 31-40 years old, and half of those with severe fatigue. No spouse, working time is more than 61 hours per week, with a disease or more related to severe fatigue. Conclusion: The factors affecting workers fatigue in high-tech industries are demographic characteristics, work characteristics and health characteristics. This result can provide to create interventions to improve health of employee in high-tech industry.