- 期刊
維生素丙對鼷鼠放射線口腔黏膜炎修復與預防效應之研究
Study on Effects of Vitamin C on Repair and Prevention of Radiation Oral Mucositis of Mice
摘要
本研究之目的在觀察BALB/c鼷鼠口腔黏膜接受放射綫照射後之病理變化，幷分析維生素丙（抗壞血酸，ascorbic acid. AA）改變這些病理變化之程度，以進一步瞭解在活體情况下，AA與放射線黏膜炎(radiation mucositis)之關係。綜合各組鼷鼠之病理變化可知，接受單次照射850cGy(2000cGy/10 Fr/2 wks)至 1050cGy (3000 cGy/15 Fr/3ks)之鼷鼠，口腔切片內出現輕微多形核細胞(polymor-phonuclear cells, PMN)浸潤。上皮部份脫落及上皮下水泡形成在1250cGy (4000cGy/20 Fr/4 wks)照射量時形成。而胞核內空泡及嚴重血管周圍及肌細胞間充血在照射量達到1579cGy(5000 cGy/25 Fr/5 wks)開始出現。至於更嚴重的上皮全部脫落則是在照射量達到1772cGy(6000cGy/30 Fr/6 wks) 開始出現。所有這些放射綫照射引起的病理變化程度如果在照射前加入AA後都會明顯減輕。本研究結果亦顯示：AA對鼷鼠口腔黏膜及其下層肌肉結締組織能减少輻射照射産生之發炎效應，其劑量調整因子(dose modification factor: DMF)值範圍由1.28至1.66。
並列摘要
The histological effects of radiations on mouse oral mucosa and its modification by ascorbic acid were studied. At a single dose range between 850cGy (equivalent to 2000cGy/10 Fr/2 wks) and 1050cGy (equivalent to 3000cGy/15 Fr/3 wks), the pathological change in oral mucosa of the BALB/c mice showed mild degree infiltration of polymorphonuclear cells (PMN). Partial epidermal drop and subepidermal blisters formation were noted at 1250cGy (4000cGy/20 Fr/4 wks). Nuclear vacuolization, severe perivascular and intermuscle cell congestion were noted after exposure to a dose of 1579cGy (5000cGy/ 25 Fr/S wks) irradiation. Furthermore, complete drop of the keratin layer was noted after 1772cGy (6000cGy/30 Fr/6 wks). All these histological effects were ameliorated when ascorbic acid was added before irradiation. It also showed that AA reduced the severity of radiation inflammation in the oral mucosa, submucosal muscle and connective tissues of mice, and the modification factor of range from 1.28 to 1.66.