- 期刊
放射性氫氧基磷灰石微粒製備及其用於肝癌治療之體外實驗
Preparation and In-Vitro Evaluation of the Radioactive Hydroxyapatite Micron-Sized Particles for Internal Radiotherapy of Hepatoma
摘要
Purpose: The radioactive hydroxyapatite micron-sized particles (RHMP) were prepared for internal radiotherapy of hepatoma. The interaction of hydroxyapatite micron-sized particles (HMP) and the cytotoxicity of RHMP to the hepatoma cells were also studied. Materials and Methods: The RHMP were synthesized using biocompatible hydroxyapatite and neutron irradiated in Tsing-Hua open pool reactor. Human hepatoma HepG2 cell line was employed for in-vitro evaluation. Results: The main nuclide of the RHMP is P-32. Cell culture tested reveals that hepatoma cells have high affinity to HMP. Tested with 70-100μm HMR the hepatoma cells climbed and surrounded the particles within 3 days. Testing with sub-micron HMP, the particles were endocytosised by hepatoma cells. Cells treated with higher dosages of RHMP showed lower surviving fraction, and cells seemed to have less morphological changes. Meanwhile, cells were treated with lower dosages, changes were observed and the RHMP could be endocytosised by hepatoma cells. Conclusion: In-vitro evaluation reveals that the RHMP are highly cytotoxic and are an effective internal radio-therapeutic agent for hepatoma. These results may have major clinical significance in the development of future research in internal radiotherapy.
並列摘要
Purpose: The radioactive hydroxyapatite micron-sized particles (RHMP) were prepared for internal radiotherapy of hepatoma. The interaction of hydroxyapatite micron-sized particles (HMP) and the cytotoxicity of RHMP to the hepatoma cells were also studied. Materials and Methods: The RHMP were synthesized using biocompatible hydroxyapatite and neutron irradiated in Tsing-Hua open pool reactor. Human hepatoma HepG2 cell line was employed for in-vitro evaluation. Results: The main nuclide of the RHMP is P-32. Cell culture tested reveals that hepatoma cells have high affinity to HMP. Tested with 70-100μm HMR the hepatoma cells climbed and surrounded the particles within 3 days. Testing with sub-micron HMP, the particles were endocytosised by hepatoma cells. Cells treated with higher dosages of RHMP showed lower surviving fraction, and cells seemed to have less morphological changes. Meanwhile, cells were treated with lower dosages, changes were observed and the RHMP could be endocytosised by hepatoma cells. Conclusion: In-vitro evaluation reveals that the RHMP are highly cytotoxic and are an effective internal radio-therapeutic agent for hepatoma. These results may have major clinical significance in the development of future research in internal radiotherapy.