- 期刊
2-Aminofluorene-Dna Adducts Formation in Rat Glial Cultured Tumor Cells
大白鼠神經膠質細胞瘤組織中2-AF-DNA的形成
摘要
目的：芳香胺化合物經N基乙醯轉移酵素N基乙醯化後可誘發癌酶症，本研究是in vitro。探討大白鼠神經膠質培養腫瘤細胞中2-AF-DNA的形成。 材料與方法：取大白鼠神經膠質培養腫瘤細胞利用乙酼化輔酶A作乙醱基及γ-[(上標 32)p]-dATP而再利用高壓層析儀分析2-AF-DNA的形成性，以aminofluorene(AF)作受質。 結果：結果大白鼠神經膠質培養腫瘤細胞有乙酼轉移酵素的活性存在，其對濃度30μM and 60μM AF為受質形成2-AF-DNA adducts各為 0.48±0.16 and 0.70±0.12pmol/mg DNA。 結論：結果發現大白鼠神經膠質培養腫瘤細胞中乙酼轉移酵素，可將2-AF乙酼化成2-AAF與細胞中去氧核糖核酸酶結合為2-AF-DNA。本研究報告是in vitro探討的初步報告，未來需進一步以in vivo的實驗確認。
並列摘要
Purpose: Arylamine N-acetylation capacity by the N- acetyltransferase (NAT) may be an important causative factor in the initiation of cancer. Most carcinogens have been demonstrated to react with cellular DNA to form covalent DNA adducts. Arylamine-DNA adducts formation have been correlated with the carcinogenic effect of heterocyclic aromatic amines. 2-AF-DNA formation in rat glial cultured tumor cell line was investigated. Materials and Methods: 2-aminofluorene-ONA (2-AF-DNA) adducts formation in rat glial cultured tumor cell line was investigated by γ-[(superscript 32)p]-dATP and HPLC, using 2-amino- fluorene as substrates. Results: 2-AF-DNA adducts formation in rat glial cultured tumor cell with 30uM and 60uM AF were 0.48±0.16 and 0.70±0.12pmol/mg DNA, respectively. Conclusion: The results indicate that rat glial cultured tumor cells activate AF to a metabolite able to bind covalently with DNA, and also induced dose-dependent effect.