不活動與維生素E補給對骨骼肌抗萎縮與氧化酶的影響

本研究以白老鼠爲受試對象，旨在探討七天吊尾的不活動與每天每公斤體重20毫克的維他命E補給對骨骼肌的重量與過氧化物歧化酶、穀胱甘肽過氧化酶、穀胱甘肽還原酶等抗氧化酶的影響。四十隻白老鼠以隨機分配方式每十隻分配至下各組：(1)不活動－維生素E補給組；(2)不活動組；(3)維生素E補給組；(4)控制組。採用二因子變異數分析考驗不活動與維生素E補給對比目魚肌的重量與過氧化物歧化酶、穀胱甘肽過氧化酶、穀胱甘肽還原酶等抗氧化酶的差異情形。結果顯示不活動組的比目魚肌重量顯著地少於(26.46%)正常活動組的比目魚肌重量；在另一方面，維生素E的補給對比目魚肌的重量並沒有造成差異。對於抗氧化酶的影響，結果指出不活動與維生素E補給對於過氧化物歧化酶、穀胱甘肽過氧化酶、穀胱甘肽還原酶沒有產生顯著性的差異。上述的發現印證了七天吊尾的不活動能够引起骨胳肌的肌肉委縮。而不活動或者維生素E補給是在低耗氧量的狀况下，因此沒有産生太多的過氧化物，也就不會促使抗氧化酶的提升。

關鍵字

不活動；維生素E補給；抗氧化酶；骨骼肌

並列摘要

Using a rat model, the effects of 7 days' immobilization induced by tail-cast suspension and vitamin E supplementation(20mg/kg/d)on tissue weight(mg)and antioxidant enzymes (U/mg protein) Superoxide dismutase (SOD), glutathione peroxidase(GPx)and glutathione reductase (GRd) in the skeletal muscle were studied. Forty Sprague-Dawley rats were grouped as follows:1)immobilization-vitamin E supplementation;2)immobilization;3)vitamin E supplementation;4)control;(10 per group).Two way ANOVA was performed to examine the effects of immobilization and vitamin E supplementation on soleus muscle weight, SOD, GPx, and GRd. The soleus muscle weight in the immobilization group was signifcantly lower (22.46%) than that in the control group; in contrast, vitamin E supplementation did not alter the soleus muscle weight. Furthermore, the immobilization and/or vitamin E supplementation had no effect on SOD, GPx, and GRd in the soleus. The findings indicate that the immobilization induced by tail-cast suspension for 7 days could result in skeletal muscle atrophy. It is concluded that immobilization and/or vitamin E supplementation may not promote increases in antioxidant enzymes due to less oxygen consumption during immobilization which decreases superoxide production.

並列關鍵字

Immobilization ； Vitamin E supplementation ； Atrophy ； Antioxidant enzyme ； Skeletal muscle

國際替代計量

不活動與維生素E補給對骨骼肌抗萎縮與氧化酶的影響

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