運動後多形核細胞內氧化壓力與去氧核醣核酸的變化情形

Alteration of Oxidative Stress and DNA Damage in PMN Leukocytes after Consecutive Intense Training

黃國欽(Kuo-Chin Huang) ； 邱亦涵(Yi-Han Chiu) ； 何采容(Tsai-Jung Ho) ； 陳詩欣(Shih-Hsin Chen) ； 徐台閣(Tai-Ger Hsu)

《體育學報》 40卷1期 (2007/03) Pp. 1-14

https://doi.org/10.6222/pej.4001.200703.1101

摘要

Polymorphonuclear leukocyte (PMN) plays a critical role in the immune defense system against infections. Oxidative damage in PMN is probably one of the mechanisms behind immune dysfunctions. Oxidative stress has been associated with strenuous exercise due to increasing aerobic metabolism and could be responsible for oxidative damage, especially intracellular DNA. Purpose: The purpose of this study was to investigate the effects of consecutive high intense exercise-induced oxidative stress on accumulation of oxidative DNA damage in PMN leukocytes. Methods: Twelve endurance subjects who performed aerobic exercise for 3 consecutive days (85 % VO2 max) and every event for 30 min were recruited to this study. Venous blood samples were collected from subjects running before (D1), immediately after Day1 (D1') and Day3 before (D3), Day3 running immediately after (D3'), Day5 (D5), and Day7 resting (D7) and respectively. Superoxide, hydroxyl peroxide (H2O2), reduced glutathione (GSH) concentrations and levels of oxidative DNA damage in PMN were measured by FACS caliber flow cytometer. Results: Superoxide is no difference in time course. To compare D1 with D3 and D1' with D3' individually; there was no different in H2O2 concentration. Intracellular GSH concentration was significantly increasing in D1' and D5, but decreasing in D7. The level of oxidative DNA damage was notably high in D3 and D3' (p＜.05). Conclusion: For 3 consecutive days (85% VO2 max) high intense exercise could probably lead to accumulation of oxidative damage in PMN leukocytes.

關鍵字

多形核細胞 ； 運動訓練 ； 氧化壓力 ； DNA傷害

並列摘要

Polymorphonuclear leukocyte (PMN) plays a critical role in the immune defense system against infections. Oxidative damage in PMN is probably one of the mechanisms behind immune dysfunctions. Oxidative stress has been associated with strenuous exercise due to increasing aerobic metabolism and could be responsible for oxidative damage, especially intracellular DNA. Purpose: The purpose of this study was to investigate the effects of consecutive high intense exercise-induced oxidative stress on accumulation of oxidative DNA damage in PMN leukocytes. Methods: Twelve endurance subjects who performed aerobic exercise for 3 consecutive days (85 % VO2 max) and every event for 30 min were recruited to this study. Venous blood samples were collected from subjects running before (D1), immediately after Day1 (D1') and Day3 before (D3), Day3 running immediately after (D3'), Day5 (D5), and Day7 resting (D7) and respectively. Superoxide, hydroxyl peroxide (H2O2), reduced glutathione (GSH) concentrations and levels of oxidative DNA damage in PMN were measured by FACS caliber flow cytometer. Results: Superoxide is no difference in time course. To compare D1 with D3 and D1' with D3' individually; there was no different in H2O2 concentration. Intracellular GSH concentration was significantly increasing in D1' and D5, but decreasing in D7. The level of oxidative DNA damage was notably high in D3 and D3' (p＜.05). Conclusion: For 3 consecutive days (85% VO2 max) high intense exercise could probably lead to accumulation of oxidative damage in PMN leukocytes.

並列關鍵字

PMN ； exercise training ； oxidative stress ； DNA damage

參考文獻

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