BPC 157 Promotes Skeletal Muscle Cells Migration in Association with Up-regulation of Paxillin and Vinculin Expression

BPC 157胜肽增加Paxillin和Vinculin蛋白表現量以促進骨骼肌細胞移行

王思恒(Szu-Heng Wang) ； 林莉萍(Li-Ping Lin) ； 林妙穗(Miao-Sui Lin) ； 蘇中慧(Jong-Hwei S. Pang) ； 蔡文鐘(Wen-Chung Tsai)

《台灣復健醫學雜誌》 47卷1期 (2019/06) Pp. 21-29

https://doi.org/10.6315/TJPMR.201906_47(1).0003

摘要

BPC 157 was first isolated from human gastric juice. It is a stable 15-amino acid fragment (GEPPPGKPADDAGLV), and the healing-promoting effects of BPC 157 have been reported on muscle injury. The aim of this study was to investigate the effect and molecular mechanism of BPC 157 on migration of skeletal muscle cells. Skeletal muscle cells were isolated from the gastrocnemius muscle of Sprague-Dawley rats, and treated with BPC 157. The cell migration was evaluated by transwell filter migration assay. The spreading of cells was evaluated microscopically. The protein expressions of paxillin and vinculin (which were positive regulators of cell migration) were assessed by Western blot analysis. The results revealed that BPC 157 could enhance migration and spreading of skeletal muscle cells. Meanwhile, the expression of paxillin and vinculin were up-regulated. It was concluded that BPC 157 promoting migration and spreading of skeletal muscle cells were associated with up-regulation of proteins expression of paxillin and vinculin.

關鍵字

BPC 157 ； skeletal muscle ； cell migration

並列摘要

BPC 157為一穩定的十五胜肽（GEPPPGKPADDAGLV），最早於人類胃酸中分離出來。目前已知BPC 157可促進受傷肌肉組織復原。細胞移行為肌肉癒合過程重要關鍵之一，本研究目的為探討BPC 157對於骨骼肌細胞移行之分子機制。利用大鼠腓腸肌分離出骨骼肌細胞，以此細胞處理BPC 157後，進行後續研究。細胞移行能力以細胞遷移實驗驗證、細胞擴展以顯微鏡觀察，並以西方墨點法驗證骨骼肌細胞中paxillin和vinculin兩種蛋白質的表現量。Paxillin和vinculin兩種蛋白質在細胞移行中擔任重要角色。研究結果顯示BPC 157促進細胞移行及擴展，且paxillin和vinculin兩種蛋白質的表現量上升。結果可知BPC 157藉由增加paxillin和vinculin兩種蛋白質的表現量，促進骨骼肌細胞移行及擴展之能力。

並列關鍵字

BPC 157胜肽（BPC 157） ； 骨骼肌細胞（skeletal muscle） ； 細胞移行（cell migration）

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