In this study, polyethylene terephthalate (PET) non-woven was pretreated using radio frequency (RF) plasma to improve surface activation, followed by grafting with acrylic acid (AAc) monomer and generating Q=C-Q group at surface. The pAAc-grafted PET was then covalently bonded with chitosan to be used as an anti-bacterial fabric surface. Modified and unmodified fabrics were characterized using Fourier-transformed infrared spectroscopy with attenuated total reflection (FTIR-ATR). Experimental result indicated that plasma-treated Aac-grafted property PET created polarizable O=C-O group, which demonstrated a hydrophilic; subsequent bonded with chitosan, the fabric surfaces provided a significant anti-bacterial (Escherichia coli and Staphylococcus aureus) effect.