利用幾丁聚醣3D多孔支架培養纖維母細胞之研究

本研究是製作幾丁聚醣多孔支架並且觀察纖維母細胞在3D多孔材料上的貼附狀態及生長情況。此3D多孔材料是以幾丁聚醣為原料，NaOH為固化劑，利用不同的轉速製成各種不同孔徑的支架。3D幾丁聚醣支架經毒性測試，其實驗結果顯示3D幾丁聚醣支架不會對細胞造成傷害。3D幾丁聚醣支架經滅菌處理後植入纖維母細胞，以石臘包埋切片、染色的方法以及SEM觀察纖維母細胞的生長狀況。SEM觀察發現纖維母細胞確實能夠生長貼附在支架上。最後針對不同孔洞大小所得到的3D幾丁聚醣支架作細胞生長曲線分析，找出最適合纖維母細胞生長之3D幾丁聚醣支架與製作條件。

關鍵字

幾丁聚醣； 3D支架；纖維母細胞；組織工程

並列摘要

This research studies the morphology and characterization of three-dimensional (3D) micro-porous structures produced from biodegradable chitosan for use as scaffolds for cells culture. The chitosan 3D micro-porous structures were produced by a simple liquid hardening method, which includes the processes of foaming by mechanical stirring without any chemical foaming agent added, and hardening by NaOH cross linking. The pore size and porosity were controlled with mechanical stirring strength. The scanning electronic microscopy (SEM) results showed that chitosan scaffold obtained by 4000 & 6000 rpm had the appropriate roughness for fibroblast attachment comparing with chitosan scaffold obtained by 2000 rpm.

並列關鍵字

chitosan ； 3D scafford ； cell culture ； tissue engineering

被引用紀錄

Chen, P. H. (2017). 亞熱帶造山帶之化學風化及其控制因子 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201703426

Teng, T. Y. (2016). 模擬台灣易山崩地區之崩塌面積及沉積物輸出 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201602168

黃致展（2011）。蘭陽溪流域系統千年來自然災變與淇武蘭文化空白之關聯〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2011.01845

Lin, Y. W. (2006). 毛細管與晶片電泳於DNA之分離及量子點之製備與光電應用 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2006.00896

Liao, S. C. (2015). 磊晶奈米結構之磁性質 [doctoral dissertation, National Tsing Hua University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0016-1802201617061465

國際替代計量

利用幾丁聚醣3D多孔支架培養纖維母細胞之研究

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