This study is based on non-woven as a substrate preparation of soilless culture medium, and using xanthan gum to increase its water absorption and water retention to improve the substrate properties. Non-woven have advantages with lightweight, easy to use and low cost. The shortcoming of non-woven water loss is improved by adding colloid, so that non-woven soilless cultivation medium suitable for cultivation plant. And discuss its water content, water retention, pH and conductivity values. From the results showed when xanthan colloid 3:1 was mixed with sodium alginate colloid, the water content of the impregnated non-woven was 89.8%, and the water retaining effect was 20 hours. The environmental pH value was maintained in the range of 5.5~7.0.