- 期刊
- OpenAccess
Production of Acteoside in Hairy-Root Culture of Paulownia fortunei Hemsl
培養泡桐毛狀根生產洋丁香酚苷
摘要
In vitro grown paulownia (”Paulownia fortunei Hemsl.”) seedlings were inoculated with ”Agrobacterium rhizogenes” (BCRC 15011; (ATCC 43057)) to produce hairy roots for acteoside (AS) production. Hairy-root lines 7-6, 23-1, and 55-1 were first cultured in MS liquid medium (50 mL flask^(-1)) to monitor their AS production, and line 55-1 was determined to have the maximal productivity (13.12±2.36 mg flask^(-1)). Subsequent examinations of this culture line revealed that MS medium and its dilutions (×1/2 and ×1/4 strength) all provided suitable nutrient components for AS production and were better than the other concentrations tested (p ＜ 0.05). It was observed that when the medium was supplemented with 60 g L^(-1) sucrose, better AS production was also achieved (p ＜ 0.05) due to enhancement of root growth. Ethanol (EtOH; 9.5％ (v/v)), a commonly used solvent for elicitor preparation, was determined to be inhibitory and caused a correspondingly low AS content when applied to the medium. A 24-h pulse treatment of methyl jasmonate (MeJA), dissolved in an appropriate concentration of EtOH and adjusted to a final concentration of 0~50 μM, was tested on a 28-d-old root culture and was noted to have had a non-significant effect on AS accumulation. Further investigation is necessary to define a better combination of pulse duration and elicitor concentration to improve AS production with this culture system.
並列摘要
In vitro grown paulownia (”Paulownia fortunei Hemsl.”) seedlings were inoculated with ”Agrobacterium rhizogenes” (BCRC 15011; (ATCC 43057)) to produce hairy roots for acteoside (AS) production. Hairy-root lines 7-6, 23-1, and 55-1 were first cultured in MS liquid medium (50 mL flask^(-1)) to monitor their AS production, and line 55-1 was determined to have the maximal productivity (13.12±2.36 mg flask^(-1)). Subsequent examinations of this culture line revealed that MS medium and its dilutions (×1/2 and ×1/4 strength) all provided suitable nutrient components for AS production and were better than the other concentrations tested (p ＜ 0.05). It was observed that when the medium was supplemented with 60 g L^(-1) sucrose, better AS production was also achieved (p ＜ 0.05) due to enhancement of root growth. Ethanol (EtOH; 9.5％ (v/v)), a commonly used solvent for elicitor preparation, was determined to be inhibitory and caused a correspondingly low AS content when applied to the medium. A 24-h pulse treatment of methyl jasmonate (MeJA), dissolved in an appropriate concentration of EtOH and adjusted to a final concentration of 0~50 μM, was tested on a 28-d-old root culture and was noted to have had a non-significant effect on AS accumulation. Further investigation is necessary to define a better combination of pulse duration and elicitor concentration to improve AS production with this culture system.