本實驗的重點在於研究抗體固定在鍍金(Au)的晶片表面的情形及電場對於抗體(IgG)固定在晶片上的影響。我們利用原子力顯微鏡(Atomic Force Microscopy; AFM)來分析其表面形貌,進而探討不同濃度下抗體固定在晶片表面的情形。另外我們也將生物分子Protein A吸附在原子力顯微鏡的探針上,利用Protein A和抗體有特殊方向的結合,來研究正負電場對於抗體固定方向的影響。
In this work, we focused on the studies of phenomenon of antibody (IgG) immobilized on gold coated chip surface under the influence of electric field. A technique of atomic force microscopy (AFM) was applied to investigate the orientation and efficiency of antibody immobilized on chip surfaces. Furthermore, protein A, an antigen which binds IgG, was adsorbed on AFM's tip and used for the specific binding study with IgG. The binding efficiency of IgG-protein A was enhanced under the influence of electric field.
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