Long duration-high intensity exercise can lead to oxidative stress, which results in the decreased immune function after exercise. The glutamine concentration has been used to reflect the status of over training and immune function after exercise. Glutamine is an amino acids, which is considered as a fuel for neutrophils and inhibit inflammation, it can also contribute to the energy recovery during a workout. Optimal glutamine concentration appears to be important to maintain neutrophils function. This paper will review findings involving the interactive influence between exercise and glutamine supplementation to neutrophils function.