This project aims to decrease the occurrence of nursing injuries caused by surgical instruments during operations. The average injury rate due to improper handling of sharp instruments for nurses in the operating room from 2008 to 2010 was 14.11 %; however, it increased to 33.33% in 2011, the highest among the 17 departments of the hospital. Through exploration and analysis of the potential causes of this result, the following problems were identified: lack of operational standards of sharp instruments, lack of knowledge in handling sharp instruments, and lack of a monitoring system. Strategies for improvement included establishing sharp instrument handling-related regulations and standards, setting up a monitoring system, and nursing in-service training. After implementation of all of the mentioned strategies, the awareness rate increased to 96.61% and the completion rate of auditing on the unit was 91.51%. The nurses' awareness in preventing injuries and properly handling sharp instruments in the operating room largely improved.