This research employs dielectrophoresis (DEP) force to separate and manipulate bio-particle s. Exchanging electro-field attempts to separate different kinds of bio-particles (yeast, cells, micro-polystyrene beads, or virus), and to manipulate particles to move simultaneously. The purpose of manipulation is moving bio-particles to next station on an integrated chip. In order to simplify experiment and observe the process, we design a platform using ISA (Industry Standard Architecture) bus slot to standardize the interface between the controller and connecter of the experimental chip. We also used software (CFD-RC) to simulate electric field distribution of the electrode array.