台灣產藥用植物山防風微體繁殖之研究

In vitro Regeneration of Echinops grijsii Hance, a Taiwan Medicinal Plant

李璟妤(Jing-Yu Li) ； 葉茂生(Mau-Shing Yeh)

《植物種苗》 10卷2期 (2008/06) Pp. 55-67

https://doi.org/10.30077/SN.200806.0004

摘要

本研究的目的在建立－有效的台灣藥用植物山防風之微體繁殖的技術。以培養的無菌苗之葉片、子葉和溫室栽培植株之幼小花及未熟子葉為培植體，在0、0.5、1 mg/L BA和0、0.1、0.3 mg/L NAA不同濃度組合之MS培養基，進行山防風癒合組織誘導及芽體再生的研究，結果如下：四種培植體在添加不同濃度BA及NAA組合之培養基均能誘導癒合組織，以幼小花之癒合組織形成率最佳，添加0.5 mg/L BA及0.1 mg/L NAA之MS培養基之形成率最高（100%）。而子葉在0.5 mg/L BA和0.3 mg/L NAA之培養基芽體的形成率最高（70%）。切取誘導之單一芽體接種在添加0.5 mg/L NAA之MS培養基能誘導根之形成，發根之小植株健化移至培養土：砂=1：1的混合介質置於溫室中栽培可大量繁殖健康的組培苗。

關鍵字

山防風（漏蘆） ； 組織培養

並列摘要

An efficient in vitro regeneration system for Echinops grijsii Hance, a Taiwan medicinal plant, has been developed. Explants of E. grijsii were excised from in vitro seedings and greenhouse grown plant. Callus cultures were initiated from lamina, young floret, mature and immature cotyledon explants on MS medium supplemented with different concentration of BA (0.5-1.0 mg/L) and NAA (0.1-0.3 mg/L). Among the different explants, the young floret explants showed the best callus formation. Maximum percentage callus (100%) formation was achieved at MS medium containing BA (0.5 mg/L) and NAA (0.1 mg/L). Maximum percentage (70%) of shoots were obtained from cotyledonary callus on MS medium containing BA (0.5 mg/L) and NAA(0.3 mg/L). Regenerated shoots rooted best on MS medium containing NAA (0.5 mg/L). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and afterwards transferred to the glasshouse.

並列關鍵字

Echinops grijsii Hance (Loulu) ； Tissue culture