Protective Effects of Oolong Tea on Oxidative Stress Induced by Exhaustive Exercise in Rat Muscle

烏龍茶對於衰竭運動後引起老鼠氧化壓力之保護

謝錦城(City C. Hsieh) ； 王宏豪(Victor H. Wang) ； 陳洸艟(Kung-Tung Chen) ； 林添鴻(Tien-Hung Lin) ； 劉介仲(Chieh-Chung Liu)

《大專體育學刊》 11卷3期 (2009/09) Pp. 105-116

https://doi.org/10.5297/ser.200909_11(3).0008

摘要

The purpose of this study was to investigate the exhaustive exercise-induced oxidative stress and the protective effect of oolong tea supplementation on malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in rat gastrocnemius muscle. Thirty-two male Sprague-Dawley rats were randomly divided into the following four groups: 1. control (C, n=8), 2. exhaustive exercise (CE, n=8), 3. oolong tea (O, n=8), 4. oolong tea-exhaustive exercise (OE, n=8). The amount of oolong tea extract supplementation was 0.5g/kg bodyweight per day for 4 weeks. The exhaustive exercise started at 10% grade, 15m min^(-1) for 10 min followed by gradual increases of treadmill speed and times until exhaustion. Two-way ANOVA was performed to examine the effects of exhaustive exercise and oolong tea supplementation on MDA, SOD, and GPx activities. The results showed that exercise-induced MDA levels were significantly higher than non-exercised rats (p＜.05), while MDA in OE group were significant lower than CE group (p＜.05). Muscle SOD activities in exercised rats were higher than non-exercised rats (p＜.05). Oolong tea supplemented rats also have higher SOD activities than non-oolong tea supplemented rats (p＜.05). Moreover, GPx activities in exhaustive exercised rats were significantly higher than non-exercised rats (p＜.05), while GPx activities in oolong tea supplemented rats were significantly higher than non-oolong tea supplemented rats (p＜.05). It is concluded that exhaustive exercise could result in oxidative stress. However, oolong tea supplementation is beneficial to decrease oxidative stress and increase the antioxidant status.

關鍵字

丙二醛 ； 過氧化物歧化酶 ； 榖胱甘肽過氧化

並列摘要

從茶葉中能夠分離出一種單酚化合物，是為茶兒素（catechin），具抗氧化作用。本研究目的旨在探討衰竭運動引起老鼠骨骼肌的氧化壓力與烏龍茶補給對老鼠丙二醛、過氧化物歧化酶及榖胱甘肽過氧化酶的活性之影響。32隻SD種雄性老鼠隨機分配到以下四組：一、控制組（C, n=8）。二、衰竭運動組（E, n=8）。三、烏龍茶組（O, n=8）。四、烏龍茶運動組（OE, n=8）。餵食濃縮烏龍茶的老鼠，其餵食量為每公斤體重0.5毫克，連續餵食四星期。從事衰竭運動的老鼠在鼠用跑步機上以坡度10%，每分鐘15公尺的速度先跑10分鐘熱身，然後逐漸增加速度與時間，直到跑至衰竭為止。以二因子變異數分析來考驗烏龍茶補給與衰竭運動對老鼠脂質過氧化物、過氧化物歧化酶、榖胱甘肽過氧化酶的活性之影響。本研究結果發現衰竭運動組的老鼠骨骼肌丙二醛活性顯著地高於控制組、烏龍茶組及烏龍茶運動組，並且達到顯著地差異（p<.05）。過氧化物歧化酶活性則是衰竭運動組顯著高（p<.05）於沒從事衰竭運動組的老鼠，並且烏龍茶補給組老鼠的過氧化物歧化酶活性顯著高（p<.05）於沒補給烏龍茶組的老鼠。至於榖胱甘肽過氧化酶方面，從事衰竭運動組老鼠骨骼肌榖胱甘肽過氧化酶的活性顯著地高（p<.05）於沒從事衰竭運動組的老鼠；也發現烏龍茶補給組老鼠的榖胱甘肽過氧化酶活性顯著地高（p<.05）於沒補給烏龍茶組的老鼠。本研究結論為衰竭運動會增加氧化壓力，而補充烏龍茶對減少氧化壓力，增加抗氧化酵素的活性有幫助。

並列關鍵字

malondialdehyde ； superoxide dismutase ； glutathione peroxidase

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