紅麴二次代謝物monascin對小鼠巨噬細胞之抗發炎作用

The anti-inflammatory Effect of the Monascus-Fermented Product Monascin on Murine Macrophages

黃韜(Tao Huang) ； 徐瑋萱(Wei-Hsuan Hsu) ； 張郁瑩(Yu-Ying Chang) ； 潘子明(Tzu-Ming Pan)

《臺灣農業化學與食品科學》 49卷3期 (2011/06) Pp. 111-117

https://doi.org/10.6578/TJACFS.2011.014

摘要

本研究利用卵白蛋白（ovalbumin, OVA）處理小鼠巨噬細胞（RAW 264.7）以誘發發炎反應，並探討紅麴二次代謝物改善發炎之機制。紅麴黃色素（monascin）及OVA共同誘導24小時後，測定細胞激素（cytokines）表現量，證實tumor necrosis factor alpha （TNF-α）及interleukin 6（IL-6）皆顯著降低；進一步觀察發炎反應下游產物之生成量，發現monascin可抑制nitric oxide synthase（iNOS）及cyclooxygenase-2（COX-2）蛋白質表現因而減少nitric oxide（NO）及prostaglandin E（subscript 2）（PGE（subscript 2））之生成，並能抑制c-Jun NH2-terminal kinase（JNK）之磷酸化程度。綜合試驗結果得知，monascin有良好之抗發炎能力，具進一步開發為抗發炎藥物之潛力。

關鍵字

紅麴 ； monascin ； 抗發炎 ； JNK

並列摘要

The present study investigated the effect of the ”Monascus”-fermented product, monascin, on inflammation caused by ovalbumin (OVA). Our purpose was to understand the anti-inflammatory mechanism of the monascin. In this study, the murine macrophage RAW 264.7 cells were co-treated with monascin and OVA for 24 hours. The results showed that cytokine expression, including tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6), was obviously decreased. We further examined the expression of the downstream products of the inflammation pathway. Monascin had effectively inhibited the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), thus decreasing the production of nitric oxide (NO) and prostaglandin E(subscript 2) (PGE(subscript 2)). It also down-regulated the phosphorylation of the c-Jun NH(subscript 2)-terminal kinase (JNK). These data suggested that monascin may have the potential to be developed as an anti-inflammation drug.

並列關鍵字

"Monascus" ； monascin ； anti-inflammation ； JNK