酸桔(Citrus sunki Hort)及扁實檸檬(Citrus depressa Hayata)果實萃取物對發炎生化指標的影響

Effects of the Sunki (Citrus sunki Hort.) and Hirami lemon (Citrus depressa Hayata) Fruit Extracts on the Inflammatory Biomarkers

陳如茵(Ru-Yin Chen) ； 蔡美珠(Meei-Ju Tsai)

《臺灣農業化學與食品科學》 49卷3期 (2011/06) Pp. 118-130

https://doi.org/10.6578/TJACFS.2011.015

摘要

The inhibitory effects of aspirin, Sunki fruit extract and Hirami lemon fruit extract on inflammation were evaluated using a mouse model with the 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment to induce inflammation on ears. Topical application of 1.0 mg aspirin/ear significantly inhibited TPA-induced increase of thromboxane B(subscript 2) (TXB(subscript 2)), prostaglandin E(subscript 2) (PGE(subscript 2)), P-Selectin and VCAM-1 by 80.08, 111, 50.23 and 73.14% (p＜0.05), respectively. The same treatment with the extract did not significantly inhibit TPA-induced edema and increase of the biomarkers associated with cardiovascular diceases, such as leukotriene B(subscript 4) (LTB(subscript 4)), proinflammatory cytokines (TNF-α, IL-1β and IL-6), E-Selectin, ICAM-1, MCP-1 and MMP-9. However, topical application of the extracts from 200 mg freeze-dried peels of Sunki (Citrus sunki Hort.) and Hirami lemon (Citrus depressa Hayata) significantly inhibited TPA-induced increase of TXB(subscript 2) levels by 84.06 and 94.71%, respectively. The effects were similar to that of 1.0 mg aspirin/ear. The extracts also significantly inhibited TPA-induced edema and increase of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6), adhesion molecules (E-Selectin, P- Selectin, ICAM-1 and VCAM-1), MCP-1 and MMP-9 (p＜0.05), except the extract of Sunki peel for E-Selectin and P-Selectin. In additional studies, there was also anti-inflammation effect in the extracts of Sunki pulps and Hirami lemon juice, but the effective dosages of the pulp and juice extracts were higher than peel extracts. The anti-inflammatory effect of the Hirami lemon peel extract was not affected after being blanched and leached.

關鍵字

阿斯匹靈 ； 酸桔 ； 扁實檸檬 ； 果皮 ； 果肉 ； 抗發炎 ； 心血管疾病 ； 生化指標

並列摘要

The inhibitory effects of aspirin, Sunki fruit extract and Hirami lemon fruit extract on inflammation were evaluated using a mouse model with the 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment to induce inflammation on ears. Topical application of 1.0 mg aspirin/ear significantly inhibited TPA-induced increase of thromboxane B(subscript 2) (TXB(subscript 2)), prostaglandin E(subscript 2) (PGE(subscript 2)), P-Selectin and VCAM-1 by 80.08, 111, 50.23 and 73.14% (p＜0.05), respectively. The same treatment with the extract did not significantly inhibit TPA-induced edema and increase of the biomarkers associated with cardiovascular diceases, such as leukotriene B(subscript 4) (LTB(subscript 4)), proinflammatory cytokines (TNF-α, IL-1β and IL-6), E-Selectin, ICAM-1, MCP-1 and MMP-9. However, topical application of the extracts from 200 mg freeze-dried peels of Sunki (Citrus sunki Hort.) and Hirami lemon (Citrus depressa Hayata) significantly inhibited TPA-induced increase of TXB(subscript 2) levels by 84.06 and 94.71%, respectively. The effects were similar to that of 1.0 mg aspirin/ear. The extracts also significantly inhibited TPA-induced edema and increase of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6), adhesion molecules (E-Selectin, P- Selectin, ICAM-1 and VCAM-1), MCP-1 and MMP-9 (p＜0.05), except the extract of Sunki peel for E-Selectin and P-Selectin. In additional studies, there was also anti-inflammation effect in the extracts of Sunki pulps and Hirami lemon juice, but the effective dosages of the pulp and juice extracts were higher than peel extracts. The anti-inflammatory effect of the Hirami lemon peel extract was not affected after being blanched and leached.

並列關鍵字

Aspirin ； Sunki ("Citrus sunki" Hort.) ； Hirami lemon ("Citrus depressa" Hayata) ； Peels ； Pulp ； Juice ； Anti-inflammation ； Cardiovascular disease ； biomarkers