Defined as an environmental force in the workplace, either real or imagined, job-related stress interacts with an individual's perception. Individuals' perceptions of job-related stress vary widely. Perceptions alter with age, gender, work experience, job characteristics, and personality behavior of individuals. In an attempt to gain a comprehensive understanding, this study examines the relationship between individual difference and the perceptions of job-related stress in the library settings of national colleges and universities in Taiwan. The result shows that individual difference is influential for librarians' perceptions of job-related stress.