Comparative Expression Profiles of Mrnas and Micrornas among Human Mesenchymal Stem Cells Derived from Breast, Face, and Abdominal Adipose Tissues

人類乳房，臉部及腹部組織來源的脂源性間葉幹細胞之基因與微核糖核酸表現分析

王凱弘(Kai-Hung Wang) ； 高安佩(An-Pei Kao) ； 沈林琥(Sher Singh) ； 俞松良(Sung-Liang Yu) ； 高立品(Li-Pin Kao) ； 蔡宗芸(Zong-Yun Tsai) ； 林幸道(Sin-Daw Lin) ； 李水龍(Shoei-Lung Li)

《The Kaohsiung Journal of Medical Sciences》 26卷3期 (2010/03) Pp. 113-122

摘要

本研究探討分別由乳房(BM19)、臉部(FM30)及腹部(AM3)組織來源的脂源性間葉幹細胞基因和微核糖核酸之表現。這三株細胞有相當類似的基因表現；利用GeneGo的分析軟體，其中有1,038個基因參與了六個與細胞黏附發展及三個與細胞骨架重塑相關的機轉。腹部(AM3)與乳房組織(BM19)而來的細胞株有39個主要的微核糖核酸表現量相似。然而，相對於腹部的AM3細胞，臉部的FM30細胞有7個表現量非常低的微核糖核酸（miR-19b，miR-320，miR-186，miR-199a，miR-339，miR-99a和miR-152）及38個微核糖核酸標的基因表現量大於腹部的AM3細胞3倍以上。因此，腹部的脂源性間葉幹細胞對乳房重建組織工程而言是一個較適合的來源，但不適合於臉部美容；此外，這38個微核糖核酸標的基因在臉部脂源性間葉幹細胞的高表現，或許在臉部組織的發展扮演著重要角色。

關鍵字

脂肪 ； 基因 ； 間葉幹細胞 ； 微核糖核酸

並列摘要

We determined the expression of both mRNAs and microRNAs (miRNAs) from human mesenchymal stem cells BM19, FM30, and AM3, which is derived from breast, face, and abdominal adipose tissues, respectively. BM19, FM30, and AM3 cells exhibited considerably similar mRNA profiles, and their 1,038 abundantly common genes were involved in regulating six cell adhesion and three cytoskeleton remodeling processes among the top ten GeneGo canonical pathway maps. The 39 most abundant miRNAs in AM3 cells were expressed at very similar levels in BM19 cells. However, seven abundant miRNAs (miR-19b, miR-320, miR-186, miR-199a, miR-339, miR-99a, and miR-152) in AM3 cells were expressed at much lower levels than that in FM30 cells, and 38 genes targeted by these miRNAs were consequently upregulated more than 3-fold in FM30 cells compared with AM3 cells. Therefore, autologous abdominal adipose-derived mesenchymal stem cells are suitable for tissue engineering of breast reconstruction because of very similar expression profiles of mRNAs and miRNAs between AM3 and BM19 cells. Conversely, abdominal AM3 cells might not be suitable for facial rejuvenation, since the 38 highly expressed genes targeted by miRNAs in FM30 cells might play an important role(s) in the development of facial tissue.

並列關鍵字

adipose ； genes ； mesenchymal stem cells ； microRNAs