A Novel Model of Left Ventricle Hypertrophy by a Complete Coarctation of the Abdominal Aorta in Rats

腹動脈完全結紮快速誘發左心室心肌肥大的動物模型探討

郭薇雯(Wei-Wen Kuo) ； 劉哲育(Jer-Yuh Liu) ； 謝逸憲(Yi-Hsien Hsieh) ； 朱嘉一(Chia-Yih Chu) ； 黃俊銘(Chun-Ming Huang) ； 朱俊憲(Chun-Hsine Chu) ； 蔡仁祥(Jen-Hsiang Tsai) ； 王朝鐘(Chau-Jong Wang) ； 徐志誠(Jyh-Cherng Shyuz) ； 杜小玲(Sheau-Ling Duh) ； 黃志揚

《中山醫學雜誌》 13卷2期 (2002/07) Pp. 137-146

摘要

This report proposes a new model for the study of cardiac hypertrophy. It involves simulated hypertension, i.e., pressure overload, meaning surgically induced through complete coarctation of the abdominal aorta at the junction of renal arteries in rats. The definite advantage of this approach is the speed with which observable results can be measured. A rapid rise in blood pressure was noticed during post-operative intervals in the week following surgery, with significant findings as early as three days post-op. Morphometric analysis of coarcted animals, when compared to sham control specimens, indicated dramatic weight increase in the left ventricle of 28% (P＜0.001) at three days following surgery and of 39% (P＜0.001) by the seventh day. Upon cross-sectional examination, left ventricular cardiomyocytes showed size expansion of 52% (P＜0.05) and 92% (P＜0.01) at three and seven days, respectively. Evidence of fibrosis was not detected during the study period. Objectives were completely successful, proving that complete coarctation in rats is a useful model for the study of cardiac pressure overload.

關鍵字

完全腹動脈結紮 ； 心肌肥大 ； 心肌細胞外型大小分析

並列摘要

This report proposes a new model for the study of cardiac hypertrophy. It involves simulated hypertension, i.e., pressure overload, meaning surgically induced through complete coarctation of the abdominal aorta at the junction of renal arteries in rats. The definite advantage of this approach is the speed with which observable results can be measured. A rapid rise in blood pressure was noticed during post-operative intervals in the week following surgery, with significant findings as early as three days post-op. Morphometric analysis of coarcted animals, when compared to sham control specimens, indicated dramatic weight increase in the left ventricle of 28% (P＜0.001) at three days following surgery and of 39% (P＜0.001) by the seventh day. Upon cross-sectional examination, left ventricular cardiomyocytes showed size expansion of 52% (P＜0.05) and 92% (P＜0.01) at three and seven days, respectively. Evidence of fibrosis was not detected during the study period. Objectives were completely successful, proving that complete coarctation in rats is a useful model for the study of cardiac pressure overload.

並列關鍵字

cardiac hypertrophy ； left ventricle ； morphometric analysis complete coarctation ； cardiomyocte ； abdominal aorta