Canine cholelithiasis is uncommon, and it is usually clinically silent. Elevated alkaline phosphatase (ALKP) and alanine aminotransferase (ALT) levels were major findings in the serum biochemical profile. Similar serum biochemical abnormalities were consisted with dogs affected with hyperadrenocoriticism. Definitive diagnosis of cholelithiasis is based upon abdominal ultrasonography. The purpose of this article was to report one dog affected with cholelithiasis and concurrent hyperadrenocoritcism. To our knowledge, cholelithiasis and concurrent hyperadrenocorticism in a dog has not been reported in veterinary literature.