Effective Eradication of Pinworm Infection (Syphacia muris, Syphacia obvelata) from a Large Rodent Breeding Center

國家實驗動物中心在89年11月每季例行健康監測時發現外圍研發組動物房SD 大鼠出現大鼠蟯蟲（Syphacia muris, 10/16），隔離區小鼠發現小鼠蟯蟲（Syphacia obvelata, 1/28），立即撲殺外圍區所有動物，並對隔離區動物開始投藥。使用十倍稀釋之1%（10 mg/mL）lvermectin混合後，裝一公升澆花瓶中噴灑鼠籠。小鼠籠噴灑三次，大鼠籠噴灑六次。噴灑頻率為每週一次，分別於第0、14、28天間隔投藥。在第7、10、21、24、35及38天時全面供應動物房piperrazine citrate, 3 g /L加於飲水中投藥，每週二次。但在投藥過程中，肛門貼片發現蟯蟲蟲卵於投藥期第10及22-23天仍出現於感染房BALB /c小鼠。所以決定於第42，49天使用0.007 mg /mL lvermectin添加於飲水中，每週一次。同時於第56-105天投藥期，大鼠使用150 ppm fenbendazole加藥飼料，小鼠使用0.2% thiobendazole加藥飼料預防再感染。其它配合措施包括感染動物房全面鼠籠加蓋，可疑污染區以噴火燄殺蟲卵，研發組動物全數撲殺，感染區減產及停止配種，經連續2年監測，總計肛門貼片撿查4862項次，腸道抹片1109項次，MIF濃縮蟲卵347項次檢查，確定驅蟲效果有效。

關鍵字

蟯蟲；大鼠蟯蟲；小鼠蟯蟲

並列摘要

In the routine health monitoring of laboratory animals in our breeding and research center, rat pinworm, Syphacia muris, was detected in the research rat colony (10/16) and mice pinworm, Syphacia obvelata, in barrier SPF mice( 1/28). A combination anthelmintic regimen was designed to eradicate this infection. At first, 0.1% ivermectin solution was sprayed in both mice and rat cages on days 0, 14 and 28, while piperrazine citrate was administered twice weekly at a rate of 3 g/L in the drinking water on days 7, 10, 21, 24, 35 and 38. Ivermectin was then added to the drinking water (0.007 mg/mL) on days 42 and 49 in both rats and mice, followed by oral administration of fenbendazole-medicated chow in rats (150 ppm) and 0.2% thiobendazolemedicated chow in mice weekly for 6 weeks. Other measures to facilitate eradication in contaminated animal rooms were conducted simultaneously and included the use of filter-top cages, environmental decontamination by flame, research colony depopulation, breeding cessation and decrease in colony size. The colony was monitored for 24 months during and after treatments. Findings from examination of 4862 cellophane-tape impressions of the anus, 1109 cecal examinations from euthanized rats and mice, as well as 347 MIF concentration stool smears confirmed the efficacy of the treatment. This is the first report for the successful eradication of pinworms in large scale breeding colonies including both rats and mice.

並列關鍵字

Pinworm ； Syphacia muris ； Syphacia obvelata

國際替代計量

Effective Eradication of Pinworm Infection (Syphacia muris, Syphacia obvelata) from a Large Rodent Breeding Center

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