台灣鵝環狀病毒之分子流行病學調查與病毒定量技術

Molecular Epidemiology and Virus Quantitation of Goose Circovirus in Taiwan

陳秋麟(Chiou-Lin Chen) ； 張伯俊(Poa-Chun Chang) ； 沈瑞鴻(Jui-Hung Shien) ； 龔順榮(Shuen-Rong Gong) ； 李敏旭(Min-Shiuh Lee) ； 陳建弘(Chien-Hung Chen) ； 謝快樂(Happy K. Shieh)

《臺灣獸醫學雜誌》 30卷2期 (2004/06) Pp. 156-162

https://doi.org/10.7009/TVJ.200406.0156

摘要

以聚合酶鏈鎖反應技術偵測台灣不同地區鵝隻屠宰場屠體華氏囊之鵝環狀病毒核酸存在陽性率，2002年間採取150個樣本中有49個樣本為陽性，陽性率為32.7%，2003年間採取237個樣本中有224個樣本為陽性，檢出陽性率高達94.5%，顯示台灣地區鵝隻感染環狀病之比率有逐年提升現象且已甚為普遍。2002-2003年間自不同地區所分離鵝環狀病毒株經全長定序及比對後發現，其序列相同性皆在97%以上，顯示台灣各地鵝環狀病毒株之基因體序列甚為穩定。以定量PCR技術檢測發現，從個別鵝隻所採集華氏囊所含之病毒套數約在5×10^6 -5×10^10 /g華氏囊之間。另外將華氏囊所含之病毒以蔗糖梯度離心純化後，病毒套數可達10^9 /μL以上，這些純化之病毒可供進一步研究如病毒致病性分析等之用。

關鍵字

鵝環狀病毒 ； 核酸序列 ； 分子流行病學 ； 聚合酶連鎖反應 ； 病毒定量

並列摘要

The goose circovirus present in bursa of Fabricius of geese collected in slaughter houses in Taiwan was detected by PCR. Among the 150 samples collected in 2002, 49 (32.7%) were found to be positive, and among the 237 samples collected in 2003, 224 (94.5%) were found to be positive. This result showed that the majority of geese in Taiwan were infected by goose circovirus and the prevalence of infection was increasing in 2002-2003. Sequence analysis of viruses isolated in 2002-2003 showed that these viruses exhibited a high degree of sequence identity (＞97%) to each other, indicating a high genomic stability of this virus. Quantitative PCR assay showed that the virus concentration in a single bursa ranged from 5x 10^6 to 5x 10^10 copies / gram of bursa and the virus could be purified by sucrose gradient centrifugation to 10^9 copies /μL. The purified virus could be used in the further studies including the pathogenicity assay of goose circovirus.

並列關鍵字

Goose circovirus ； Molecular epidemiology ； Nucleotide sequence polymerase chain reaction(PCR) ； Virus quantitation