奈米銀凝膠對糖尿病大鼠皮膚傷口癒合效應之研究

The Study of Topically Applied Nanonized Silver Gel Enhances Wound Healing in Diabetic Rat Skin

趙安基(An-Ji Chao) ； 丘宏治(Andy Hong-Jey Chiou) ； 蔡清恩(Chin-En Tsai)

《臺灣獸醫學雜誌》 37卷1期 (2011/03) Pp. 36-44

https://doi.org/10.7009/TVJ.201103.0036

摘要

The purpose of this study is to investigate the effects of nanonized silver gel (nAg gel) on the delayed wound healing in diabetic rats. Adult male SD rats (BW: 375±25g) were divided at random into 4 groups (A, B, C and D, six rats per group), Groups A and B, and Groups C and D were injected intraperitoneally with 0.1 mol/L citrate buffer, and streptozotocin (STZ, 70 mg/kg! week), respectively. Six (1×1 cm^2) full-thickness skin wounds in the dorsal thorax (3 on each side) in each rat were created. Nondiabetic rats were control groups (group A: wounds treated with gel, 50 μL/wound; group B: wounds treated with nAg gel, 50 μL/wound). Diabetic rats were divided into 2 experimental groups (group C: wounds treated with gel, 50 μL/wound; group D: wounds treated with nAg gel, 50 μL/wound). On the 0, 3(superscript rd) 5(superscript th) 7(superscript th) and 14(superscript th) day and 21(superscript st) day, each of the wound healing areas was measured and the histopathological examination (light microscope and electric microscope) was observed. Results: 1. Poor wound healing in diabetes mellitus was characterized by decreased wound collagen content. 2. The value of blood glucose in diabetic rats was steadily higher than 300 mg/dL. 3. The body weight in diabetic rats was lighter than that in normal rats. 4. In nAg gel-treated rats, the wound healing areas and re-epithelialization were greater than that in gel-treated rats (e＜0.05). Nanonized silver gel maybe a valuable adjunct for chronic wounds, including diabetic and other non-healing chronic ulcers.

關鍵字

糖尿病鼠 ； 傷口癒合 ； 奈米銀凝膠

並列摘要

The purpose of this study is to investigate the effects of nanonized silver gel (nAg gel) on the delayed wound healing in diabetic rats. Adult male SD rats (BW: 375±25g) were divided at random into 4 groups (A, B, C and D, six rats per group), Groups A and B, and Groups C and D were injected intraperitoneally with 0.1 mol/L citrate buffer, and streptozotocin (STZ, 70 mg/kg! week), respectively. Six (1×1 cm^2) full-thickness skin wounds in the dorsal thorax (3 on each side) in each rat were created. Nondiabetic rats were control groups (group A: wounds treated with gel, 50 μL/wound; group B: wounds treated with nAg gel, 50 μL/wound). Diabetic rats were divided into 2 experimental groups (group C: wounds treated with gel, 50 μL/wound; group D: wounds treated with nAg gel, 50 μL/wound). On the 0, 3(superscript rd) 5(superscript th) 7(superscript th) and 14(superscript th) day and 21(superscript st) day, each of the wound healing areas was measured and the histopathological examination (light microscope and electric microscope) was observed. Results: 1. Poor wound healing in diabetes mellitus was characterized by decreased wound collagen content. 2. The value of blood glucose in diabetic rats was steadily higher than 300 mg/dL. 3. The body weight in diabetic rats was lighter than that in normal rats. 4. In nAg gel-treated rats, the wound healing areas and re-epithelialization were greater than that in gel-treated rats (e＜0.05). Nanonized silver gel maybe a valuable adjunct for chronic wounds, including diabetic and other non-healing chronic ulcers.

並列關鍵字

diabetic rat ； nanonized silver gel ； wound healing