Nerve Re-innervations by an End-to-side Neurorrhaphy of Myocutaneous Transplantation in Rats

末端神經側邊吻合術於大鼠肌肉皮膚移植之神經再生研究

鄭穹翔(Chiung-Hsiang Cheng) ； 古雲鳳(Yun-Feng Ku) ； 侯君翰(Chun-Han Hou) ； 葉力森(Lih-Seng Yeh) ； 張芳嘉(Fang-Chia Chang)

《臺灣獸醫學雜誌》 37卷3期 (2011/09) Pp. 143-151

https://doi.org/10.7009/TVJ.201109.0143

摘要

末端對側邊神經吻合術最早被記載於19世紀，但那時並沒有廣泛被使用。此研究的目標為評估利用末端對側邊神經吻合術於大鼠自體移植及異體移植肌皮膚的神經再生效果。移植方法為於捐贈者取－含有薄肌(gracilis muscle)及閉孔神經(obturator nerve)的移植瓣藉由末端對側邊神經吻合術移植到接受者的閉孔神經上。評估神經再生性的方法為刺激肌肉的收縮能力、組織型態學觀察及計算切面的神經軸突數量。結果顯示神經於執行末端對側邊神經吻合術後皆有再生情形。接受者神經末梢軸突計算方面，具有橫切面的組別與沒有橫切面的組別以及自體移植跟異體移植的組別皆沒有統計學上的差異。於自體移植跟異體移植的組別都可見肌肉功能上的恢復。此外，免疫抑制的情形並沒有影響執行末端對側邊神經吻合術後的異體移植組別。這些結果顯示出神經再生性及功能恢復性可藉由末端對側邊神經吻合術於自體移植及異體移植上恢復。

關鍵字

異體移植 ； 末端對側邊神經吻合術 ； 自體移植

並列摘要

End-to-side (terminolateral) neurorrhaphy was first documented in the 19(superscript th) century, but it hasnt been routinely practiced. The purpose of this study is to evaluate nerve reinnervation after isogeneic or allogeneic end-to-side neurorrhaphy in a novel rat myocutaneous model. The obturator nerve with a gracilis muscle island flap from the donor was sutured end-to-side to the obturator nerve of the recipient through an epineurial window. The obturator nerves of the recipients distal to the neurorrhaphy site were either intact or severed. The evaluation of nerve reinnervation was dependent on the stimuli-induced muscle contraction, the histological and morphological observations, and the counting of nerve axons in a cross section. Results revealed that nerve reinnervation crossing the end-to-side neurorrhaphy sites was significant in all experimental groups. The axon counts of the receiving nerves distal to the neurorrhaphy sites were not statistically different between groups with transection and without transection of the distal nerve, and neither iso-transplantation nor allotransplantation exhibited difference. Functional recovery could be observed after both iso-and allotransplantations. In addition, immunosuppression did not affect the end-to-side nerve reinnervation in the allograft group. This result indicates that nerve reinnervation and functional recovery could be restored by the end-to-side neurorrhaphy of receiving either iso-or allo-transplantation.

並列關鍵字

Allo-transplantation ； end-to-side neurorrhaphy ； iso-transplantation