假性狂犬病毒細胞培養最佳增殖條件之探討

為了解假性狂犬病病毒（pseudorabies virus；PrV）於RK-13（rabbit kidney cell）、CRFK（Crandell feline kidneycell）、PK-15（porcine kidney cell）、BHK-21（baby hamster kidney cell）四種不同細胞株內增殖之力價差異，分別以0.1、0.01、0.001 MOI（multiplicity of infection）之PrV接種上述細胞株，並於攻毒後第一、二及三天收集病毒並測定力價。結果顯示，PrV可於此四種細胞株產生顯著之細胞病變，但於不同細胞株有顯著的不同；在所有測試條件中，以0.01 MOI接種PK-15二天後，可達最高力價（10^8.09 TCID50/0.1 mL）；而以0.1 MOI接種CRFK一天後，PrV力價僅為10^5.70 TCID50/0.1 mL。綜而論之，PrV對多種細胞株皆具感受性，然其最適增殖條件，於不同細胞株間有顯著之不同。於本實驗中以PK-15為假性狂犬病病毒增殖之理想細胞株。

關鍵字

細胞株；感染複數；假性狂犬病病毒；病毒力價

並列摘要

This study determined the specific parameters affecting in vitro propagation titers of pseudorabies virus (PrV). The four cell lines used in this series test included a rabbit kidney cell (RK-13), a Crandell feline kidney cell (CRFK), a porcine kidney cell (PK-15), and a baby hamster kidney cell (BHK-21). The cells were infected with varying MOI (0.1, 0.01, and 0.001) of PrV and harvested at 1, 2, and 3 days post-infection (DPI). Viral titers of each sample were estimated using tissue culture infection dose determination. Cytopathic were clearly visible in four cell lines after PrV infection. However, susceptibility significantly differed among cell lines. In all treatments, the highest titer was obtained in PK-15 infected with 0.01 MOI of PrV and harvested at 2 DPI (10^8.09 TCID50/0.1 mL). In contrast, the lowest titer was obtained in CRFK infected with 0.1 MOI of PrV and harvested at 1 DPI (10^5.70 TCID50/0.1 mL). These experimental results show that PrV replicates in four tested cell lines and that PK-15 provide optimal PrV propagation.

並列關鍵字

cell line ； MOI ； pseudorabies virus ； viral titer

國際替代計量

假性狂犬病毒細胞培養最佳增殖條件之探討

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