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雲嘉南地區山羊流產披衣菌(Chlamydophila Abortus)的疫情及傳播

The Epidemic and Spread of Chlamydophila Abortus in Goats from the Yun-Chia-Nan Region in Taiwan

摘要


流產披衣菌(Chlamydophila abortus;Cp.abortus)可導致山羊流產,是人畜共通之病原;雲嘉南地區是國內山羊飼養的重鎮,本研究自2008年至2011年調查雲嘉南地區山羊Cp. abortus的疫情,並探討其傳播。6個羊場血清披衣菌屬抗體陽性率為60%(18/30),19場Cp. abortus抗體陽性率為9.3%(38/409);以PCR檢測流產胎兒組織,Cp. abortus檢出率最高為26.1%(6/23);故Cp. abortus應是山羊流產的主因,但羊群應有他種披衣菌感染。羊場內陰道棉拭樣本檢測Cp. abortus傳播分析發現,未曾發生流產病例場母羊檢出率0%,顯著低於曾經發生流產場;一年前曾流產場顯著低於一週內流產場;羊流產時與牠同欄飼養的孕羊顯著高於未接觸流產羊之待淘汰欄的母羊;三組比較之p值皆小於0.05,故感染之Cp. abortus山羊於流產時之排菌及病原接觸傳播最為嚴重。所有分離株序列相似性在97.4-100%之間,可區分為I-V相似菌株族群(cluster),僅085一株種系較接近ovine S26/3,complete genome.AF111200.1。因此,批衣菌發生場,與流產母羊同欄舍飼養的母羊有被高度傳染風險。披衣菌引起的流產胎兒,肝、脾及胎盤可作為採樣分離的標的。血清學監測及配合陰道棉拭樣本檢驗快速、簡便及敏感性高,可作為該菌早期診斷的方法。

關鍵字

山羊 披衣菌 陰道棉拭 ELISA PCR

並列摘要


Chlamydophila abortus (Cp. abortus) is a zoonotic pathogen which can lead to abortion in goats. The Yunlin-Chiayi-Tainan region is an important domestic goat rearing area. An epidemiological investigation of Cp. abortus in goats and its mode of transmission were studied between the year 2008 and 2011. The sero-positive rate of Cp. abortus in goats collected from six farms was 60% (18/30), while the sero-positive rate of Cp. abortus detected from 19 farms was 9.3% (38/409). Aborted fetuses were also checked for the presence of six abortigenic pathogens by a polymerase chain reaction technique; Cp. abortus had the highest detection rate of 26.1% (6/23). These findings suggested Cp. abortus that should be the main cause of abortion in goats but infections by other species of Chlamydia were also considered. Using virginal swabs for Cp. abortus detection and analysis revealed that the detection rate in farms without abortion history was 0%, which was obviously lower than the rates in farms having abortion history. The detection rates in the farms, which reported the last abortion case one year ago, were significantly lower than the rates in the farms, which had abortion cases within a week before the study began. The detection rate in the pregnant does, which lived with the does just having abortion, was significant higher than that in the pre-culling does without abortion history, which did not live with the does just having abortion. The P values are all < 0.05 in all three above comparisons; which reveal that goats infected by Cp. abortus and just had a story of abortion recently can disseminate the pathogen massively and result in the well establishment of the transmission route. The genetic similarities among all isolated strains are between 97.4% and 100% and the strains can be sorted into the I to V cluster. Only the strain 085 is phylogenetically closest to ovine S26/3, complete genome. AF111200.1.Therefore, in Chlamydia infected farms, the does rearing together with other aborting does have high risk of getting the pathogen. The target organs for detecting the pathogen from aborted fetuses are liver, spleen and placenta. Serum surveillance methods and virginal cotton swabs are easy, sensitive and rapid tools to detect pathogens and then, they are suggested to perform preliminary diagnoses of Chlamydia.

並列關鍵字

chlamydia ELISA goats PCR vaginal cotton swab

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