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台灣雲嘉南地區流浪犬壁蝨媒介性疾病之調查

Epidemiological Survey of Tick-borne Disease of Stray Dogs in Yun-Chia-Nan Areas in Taiwan

摘要


犬壁蝨媒介性傳染病為一個持續受到重視的人畜共通傳染疾病,由於單一種壁蝨可作為許多不同病原的病媒,因而在流行區域內犬隻可能發生多種病原共同感染(coinfection) 的情形。本研究自2010年4月至2011年3月於雲嘉南地區採集收容所犬隻血液,進行血液塗抹片檢查、血清學檢驗及Ehrlichia canis、Babesia spp.、Hepatozoon canis、Anaplasma phagocytophilum與Borrelia burgdorferi sensu lato之聚合酶鏈鎖反應(polymerase chain reaction; PCR)之調查。血液塗抹片檢查結果有2個樣本於顆粒球內發現艾利希體之桑椹體,有2個樣本於紅血球內觀察到焦蟲蟲體,有5個樣本於白血球內發現肝簇蟲之配子體,皆未在單核球內發現艾利希體之桑椹體。血清學檢驗之檢出率分別為14.3% (Dirofilaria immitis)、6.3%(E. canis)、14.3%(A. phagocytophilum)及0%(B. burgdorferi sensu lato);而PCR檢測之檢出率分別為0%(E. canis)、17.2%(A. phagocytophilum)、1.4%(B. burgdorferi sensu lato)、8.1%(Babesia spp.)以及5.6%(H. canis),其中Babesia spp.有93%為B. canis。本研究結果顯示,雲嘉南地區犬壁蝨媒介性傳染病以A. phagocytophilum感染為主;而PCR則是敏感性較高之診斷方式。

關鍵字

壁蝨 艾利希體 焦蟲 肝簇蟲 伯氏疏螺旋體

並列摘要


Canine tick-borne infectious disease is a considerable zoonosis, notably Babesiosis, Ehrlichiosis, Hepatozoonosis and Lyme disease. The same tick species can be a vector for several pathogens, therefore, dog coinfected with different tick-boane pathogens may occur in endemic areas. The present study was conduct to survey tick-borne infectious of stray dogs in Yun-Chia-Nan areas. Blood was collected in the period from April 2010 to March 2011. The bloods were tested by blood smear, serological test and PCR of Ehrlicha canis, Babesia spp., Hepatozoon canis, Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato., Babesial organism. In blood smear, Hepatozoonal gamont was detected in leukocytes in 5 dogs, Babesial organism was detected in red blood cell in 2 dogs, Ehrlichial morula was only detected in graulocyte in 2 dogs. The prevalence of serological test of Dirofilaria immitis, E. cans, A. phagocytopilum and B. burgdorferi sensu lato were 14.3% (18/126), 6.3% (8/126), 14.3% (18/126) and 0%, respectively. The prevalence of PCR of, E canis, A. phagocytophilum, B. burgdorferi sensu lato, Babesia spp. and H. canis were 0%, 17.2% (62/360), 1.4% (5/360), 8.1% (29/360), and 5.6% (20/360), respectively. In conclusion, the main canine tick-borne disease in Yun-Chia-Nan areas is A. phagocytophilum, and the PCR is a more sensitive method for diagnosis of tick-borne infectious disease.

並列關鍵字

canine tick Ehrlichia Babesia Hepatozoon B. burgdorferi

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