病例報告：火雞組織鞭毛蟲與腸炎沙門氏菌混合感染症

台南縣某貝茨維爾小型白色火雞（Beltsville turkey）飼養場（1,000羽），於2012年2月底，發現55日齡火雞於臨床上呈現雞冠顏色變黑及生長遲緩的情形。該場本次發病率與死亡率分別約佔總飼養隻數的11%與8.1%。剖檢火雞肉眼病變，分別於肝臟呈現腫大且表面有多發局部菊花樣病灶，及盲腸可見腫脹及觸感稍堅實；細菌學檢查結果於肝臟組織分離之細菌經由分子生物學鑑定為腸炎沙門氏菌；組織病理學檢查結果於肝臟呈現大區域與多發局部壞死，且壞死區內可見多核巨大細胞，巨噬細胞吞噬之嗜伊紅性原蟲體，盲腸黏膜層至漿膜層有嚴重壞死出血、多核巨大細胞及巨噬細胞之嗜伊紅性原蟲體；分子生物學檢查使用聚合酶鏈鎖反應及巢氏聚合酶鏈鎖反應針對組織鞭毛蟲之核醣體小次單元核苷酸片段進行增幅結果為陽性。因此，依據臨床症狀、肉眼及組織病理變化與實驗室檢查結果，確診本病例為火雞組織鞭毛蟲與腸炎沙門氏菌混合感染症。

關鍵字

組織鞭毛蟲感染症；腸炎沙門氏菌；火雞

並列摘要

In February 2012, a farm raising approximately one thousand small white Beltsville turkeys at Tainan city was reported to have 55-day old turkeys clinically presented with blackening combs and retarded growth. The morbidity and case fatality rates were 11% and 8.1%, respectively. The macroscopic lesions noted were enlarged livers with multifocal round and radial/radiating lesions and swollen cecum of solid consistency on palpation. In microbiological tests, bacteria isolated from liver tissues were identified as Salmonella enterica serovar Enteritidis using molecular biology methods. The histopathology demonstrated extensive and multifocal necrosis in liver with multinucleated giant cells seen in the necrotic areas and macrophages engulfing eosinophilic protozoans. In cecal mucosa and serosa, severe hemorrhagic necrosis was also noted with multinucleated giant cells and macrophages engulfing eosinophilic protozoans. PCR and nested PCR amplified a particular nucleotides sequences belonging to small subunit of ribosome from Histomonas. Evidence from clinical signs, macro- and histo-pathologiy and laboratory tests suggest a final diagnosis as concurrent infection of Histomonas meleagridis and Salmonella Enteritidis in turkeys.

並列關鍵字

Histomonas meleagridis ； Salmonella Enteritidis ； turkey

國際替代計量

病例報告：火雞組織鞭毛蟲與腸炎沙門氏菌混合感染症

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