Thermography, a noninvasive diagnostic method, can rapidly document heat related to diseases and be helpful in disease diagnosis, treatment, and prognosis. In this study, infrared thermography was assessed in clinical application in captive bottlenose dolphins. There were four cases reported. Two were that abnormal thermogram was noticed without any abnormality in visual observation. The other two were that the thermogram turned back to normal before normal condition of skin was observed during treatment. This study showed that thermography can be applied as an efficient and convenient clinically diagnostic imaging method in dolphins. Thermography describes the inflammation or active infection, and this information can allow the clinician to prevent more serious injury and begin rapid adjustment of therapy.