Background and Purpose: The goal of percutaneous breast core needle biopsy (CNB) is to allow accurate planning of a single stage definitive surgery. The purpose of this study is to evaluate the underestimation rate of ultrasound (US)-guided core needle biopsy (CNB) in detection of the breast lesion. Methods: 283 cases received CNB under US guidance were analyzed retrospectively. 81 patients underwent additional surgery. These included 16 cases of high risk group, 11 cases of ductal carcinoma in situ (DCIS) and 54 cases of invasive carcinoma. Results: 3 of the 16 patients with high risk lesions by CNB were upstaged to carcinoma and 3 of the 11 patients with DCIS were discovered with occult invasion. There is no false positive case in our study. Conclusion: We further confirmed US detected breast lesion can be easily assessed by US-guided biopsy. CNB is a reliable and easily performed method without significant complications. The effective communication of radiologist, surgeon and pathologist is critical for the success of an interventional breast biopsy program.