This study explored the relationship between the large vascular bundle number of panicle neck internode and the branching structure of panicles. Experiment material contained Japonica and Indica rice cultivars with different grain appearances. The large vascular bundle number of panicle neck internode was higher in Indica cultivars than in Japonica cultivars. There was a significant positive correlation between and number of large vascular bundles in panicle neck internode and panicle weight and spikelet number per panicle. As a result, the data indicate that an increase of the number and area of panicle vascular bundle may benefit the increase of panicle spikelet number and panicle weight. However, the increase of tertiary vascular branch number could enhance the appearance of the trait of white-belly. Thus, milling quality may be improved by reducing tertiary number.