Previous studies have examined the relationship between job satisfaction and service quality. However, little research examined such a relationship among employees in international hotels. Moreover, by reasoning, gender may have a moderating effect on that relationship. Therefore, the purpose of this study is to investigate the relationship of job satisfaction and gender to service quality. Using survey questionnairs, the sample included 221 employees in 10 Taiwan international hotels. Hierarchical regression analysis was used for data analysis. Empirical data showed that job satisfaction to be positively related to service quality. However, the moderating effect of gender was not supported. Theoretical and practical suggestions are also provided.