Authentication of Actinidia macrosperma Using PCR-RFLP Based on trnK Sequences

貓人參是瀰猴桃屬植物大籽瀰猴桃(Actinidia macrosperma)的根莖，是中國華東地區常用的抗腫瘤中草藥。同屬的鑷合瀰猴桃(A. valvata)。黑蕊瀰猴桃(A. melanandra)等常被混用作貓人參，嚴重混淆了貓人參正品的種源，危害了貓人參的藥效。然而大籽瀰猴桃及其混淆品原植物的鑒別較困難，營養生長期尤難鑒別。本文探用PCR-RFLP法，用限制性內切酶DdeI。DraI消化葉綠體trnK序列，獲得了大籽瀰猴桃的特異性消化式樣，可作為鑒別貓人參正品種源大籽瀰猴桃的分子標記，用於貓人參的GAP生產。進一步的序列比對結果表明，供試材料序列位點變異均為單一鹼基顛換或轉換，不存在鹼基或片段的插入或缺失，大籽瀰猴桃有11個點突變位點。

關鍵字

大籽獼猴桃；鑷合獼猴桃； PCR-RFLP ；分子標記

並列摘要

”Cat ginseng”, the dried root of Actinidia macrosperma, is a famous traditional Chinese medicine against cancers in eastern China. The roots of some other species of the genus Actinidia such as A. valvata and A. melanandra have also been used as fake ”cat ginseng”, but they have little therapeutic value. However, identification of the original plants used to make the crude drugs is difficult, especially during the vegetation period. In this study we developed molecular markers for the determination and authentication of A. macrosperma. The restriction digestion of the chloroplast trnK region using endonucleases DdeI and DraI produces two unique patterns in A. macrosperma, and in the matK sequences, there are 11 sites unique to A. macrosperma. The molecular markers provide an effective and accurate identification and authentication of A. macrosperma in Actinidia.

並列關鍵字

Actinidia macrosperma ； Actinidia valvata ； PCR-RFLP ； Molecular marker

國際替代計量

Authentication of Actinidia macrosperma Using PCR-RFLP Based on trnK Sequences

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