Forty endophytic fungal isolates were obtained from symptomless leaf samples of nine Taxus mairei trees on Fu-Shan, Taiwan. Identification was based on morphological characters and comparison of rDNA ITS sequences to those in GenBank. Colletotrichum and Fusarium were the endophytic fungi most frequently isolated from leaves of T. mairei. Colletotrichum gloeosporioides was newly recorded as an endophyte of T. mairei.