Isolation and Characterization of Microsatellite loci in Ajuga Taiwanensis Nakai ex Murata Using PCR-based isolation of Microsatellite Arrays (PIMA)

利用PIMA方法分離及分析台灣筋骨草微衛星基因座

黃琮竣(Tsurng-Juhn Huang) ； 陳怡雁(Yi-Yen Chen) ； 王國雄(Kuo-Hsiung Wang) ； 謝宗欣(Tsung-Hsin Hsieh) ； 周昌弘(Chang-Hung Chou)

《Botanical Studies》 50卷1期 (2009/01) Pp. 21-24

摘要

台灣筋骨草(Ajuga taiwanensis)為具有價值之傳統中草藥。此研究從台灣筋骨草分別分離出8個微衛星基因座，這些微衛星基因座用來篩選台灣筋骨草15個野生族群個體以及兩個同屬不同種A. bracteosa及A. nipponensis，在台灣筋骨草，對偶基因數目範圍為3到11個，異質度觀測值(H(下標 O))以及期望值(H(下標 E))範圍分別為0.54253到0.90575以及0到1.0。所有分析之基因座因為異型合子差異性而顯著偏離Hardy-Weinberg預估值，顯示侷限分佈物種有明顯遺傳多型性之缺失。這些在三種筋骨草皆能成功增幅之基因座可以用來分析物種遺傳多樣性及族群遺傳結構並且可以對這些瀕危物種建立保育策略及提供有用之資訊。

關鍵字

台灣筋骨草 ； 日本筋骨草 ； 遺傳歧異度 ； 微衛星基因座

並列摘要

Ajuga taiwanensis is a valuable herb for traditional Chinese medical treatment. In this study, eight microsatellite loci from A. taiwanensis were isolated. The simple sequence repeat (SSR) markers were screened in 15 samples of wild populations of A. taiwanensis, and nine samples from its sister A. bracteosa and A. nipponensis. In A. taiwanensis, the number of alleles ranged from 3 to 11, and values of expected (H(subscript E)) and observed (H(subscript O)) heterozygosity were 0.54253-0.90575 and 0.0000-1.0000, respectively. All loci deviated significantly from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed species. The markers amplifying well in the three species are useful for examining genetic diversity and population genetic structure, and this can provide information for establishing a conservation strategy for these endangered species.

並列關鍵字

Ajuga nipponensis ； Ajuga taiwanensis ； Heterozygosity ； Microsatellite ； PIMA