Soil Bacterial Community Composition Across Different Topographic Sites Characterized by 16S rRNA Gene Clones in the Fushan Forest of Taiwan

利用16S核糖體基因選殖株分析台灣福山森林不同地形位置土壤細菌組成

蔡書憲(Shu-Hsien Tsai) ； 韓亞盤(Ammaiyappan Selvam) ； 張鈺苹(Yu-Ping Chang) ； 楊盛行(Shang-Shyng Yang)

《Botanical Studies》 50卷1期 (2009/01) Pp. 57-68

摘要

本研究經由聚合酵素鏈鎖放大反應、選殖及變性梯度凝膠電泳分析基因體DNA中的16S rDNA，探討台灣福山森林溪谷、中坡及稜線土壤細菌組成。演化分析顯示九個基因選殖庫中的所有選殖株可分為Proteobacteria、Acidobacteria，Actinobacteria、Bacteroidetes、Cyanobacteria、Firmicutes、Gemmatimonadetes、Nitrospirae、Planctomycetes、candidate division TM7和Verrucomicrobia等族群。其中Proteobacteria、Acidobacteria和Actinobacteria分別佔全部選殖基因庫的49.1%、32.3%和6.3%，而其他的族群則皆未超過6%。稜線地區有最多的細菌種類，顯示地形影響。有機質層比下層土壤細菌組成多樣化。細菌種類數量亦隨不同土層而改變。

關鍵字

16SrDNA選殖基因庫 ； Acidobacteria ； 細菌組成 ； 變性梯度凝膠電泳 ； Proteobacteria ； 地形

並列摘要

Bacterial communities present in soils from the valley, middle-slope, and ridge sites of the Fushan forest in Taiwan were characterized using 16S rDNA analysis of genomic DNA after polymerase chain reaction amplification, cloning, and denaturing gradient gel electrophoresis analysis. Phylogenetic analysis revealed that the clones from nine clone libraries included members of the phyla Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, candidate division TM7, and Verrucomicrobia. Members of Proteobacteria, Acidobacteria, and Actinobacteria constituted 49.1%, 32.3%, and 6.3% of the clone libraries, respectively, while the remaining bacterial divisions each comprised less than 6%. The ridge site exhibited the most bacterial species number, indicating the influence of topography. Bacterial composition was more diverse in the organic layer than in the deeper horizons. In addition, bacterial species numbers varied across the gradient horizons.

並列關鍵字

16S rDNA library ； Acidobacteria ； Bacterial community ； DGGE ； Proteobacteria ； Topography