本研究係針對大豆種子的晚胚蛋白在乾燥的環境下,對人造微脂體的保護效果。同時,我們也以由晚胚蛋白與蔗糖所組成的玻璃質體,進行微脂體保護效果的研究。結果顯示,屬於第二群晚胚蛋白的GmPM6 對於乾燥的微脂體具有明顯的保護效果,但第四群的晚胚蛋白GmPM16 則較低。另一方面,無論GmPM6 或GmPM16 與蔗糖所組成的玻璃質體皆無法對乾燥的微脂體產生足夠的保護能力。因此,雖然晚胚蛋白與微脂體之間能夠產生靜電吸引力與氫鍵,但維持微脂體的完整性可能是由於其它的作用力。此外,關於成熟的正儲型種子的細胞玻離質體的功能也加以討論
Immature seeds can acquire the traits of desiccation tolerance and germination after precociousmaturation. This process is associated with the accumulation of non-reducing sugars and late embryogenesis abundant (LEA) proteins. LEA proteins were first identified from late developmental seeds and were proposed to be associated with desiccation tolerance. In the present study, we examined whether purified LEA fusion proteins from soybean seeds can maintain the integrity of artificial liposomes under desiccation. We also studied the relationship between the protective effects of LEA protein-sucrose glasses and desiccated membrane. GmPM6 (LEA II) but not GmPM16 (LEA IV) provided significant protection to dehydrated liposomes. However, neither GmPM6- nor GmPM16-sucrose glasses could protect these liposomes. We discuss the functions of cellular glass in mature orthodox seeds.
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