Photosynthetic Properties and Photosystem Stoichiometry of in Vitro-Grown Juvenile, Adult, and Rejuvenated Sequoia Sempervirens (D. Don) Endl.

紅杉試管幼年、老齡、及其復幼植莖光合作用特性和光系統比例改變之研究

朱修安(Hsiu-An Chu) ； 張英峯(Ing-Feng Chang) ； 沈金輝(Chin-Hui Shen) ； 陳玉婷(Yu-Ting Chen) ； 王幸婷(Hsing-Ting Wang) ； 黃麗春(Li-Chun Huang) ； 葉開溫(Kai-Wun Yeh)

《Botanical Studies》 53卷2期 (2012/04) Pp. 223-227

摘要

本研究主要探討紅杉不同的生長相（幼年期、成齡期、及返老還輕微後）的植莖光合作用特性和光系統比例的變化情形。發現成齡老株的葉綠體中的光系統二對應光系統一的比例和光合作用釋放氧氣的速率明顯的高於幼年莖和復幼莖。顯示紅杉的葉綠體中光系統的比例在不同的生長相有顯著的變化。雖然幼年期或成齡期的光合系統的電子傳遞效率（F（下標 m）-F0）/F（下標 m）並無明顯的差異, 但是成齡老株AS76的光合作用有明顯較高的熱消散（Nonphotochemical quenching；NPQ）現象。這些光合作用特性和光系統比例的變化可能反映了紅杉不同的生長相間的生理狀態的差異。

關鍵字

紅杉 ； 生長相 ； 光合作用 ； 光系統比例 ； 熱消散

並列摘要

Photosynthetic properties and photosystem stoichiometry of in vitro-grown juvenile, adult, and rejuvenated Sequoia sempervirens shoots were characterized. 77K fluorescence analysis indicated that photosystem II/photosystem I ratios were highest for chloroplasts in adult shoots. Photosynthetic oxygen evolution rates (on the same chlorophyll bases) were also slightly higher for chloroplasts in adult shoots. Our results suggested a significant alteration of photosystem stoichiometry in chloroplasts of Sequoia sempervirens shoots during phase change. In addition, chlorophyll a fluorescence analysis showed that juvenile, adult, and rejuvenated shoots showed virtually identical maximal quantum efficiencies of photosystem II (F(subscript V)/F(subscript M)). Nonphotochemical quenching (NPQ) during actinic light illumination, however, was significantly enhanced for AS76 adult shoots. The differences in photosynthetic properties and photosystem stoichiometry among juvenile, adult, and rejuvenated shoots may reflect adjustments in the photosynthetic apparatus to acclimate to distinct physiological states of Sequoia sempervirens during phase changes.

並列關鍵字

Nonphotochemical quenching ； Phase change ； Photosystem stoichiometry ； Photosynthesis ； Sequoia sempervirens