芭菲爾鞋蘭植物染色體核型分析之研究

The Research on Karyotype Analysis of Paphiopedilum

劉黃碧圓(Pi-Yuan Liu Huang)

《臺灣園藝》 57卷1期 (2011/03) Pp. 19-29

https://doi.org/10.6964/JTSHS.201103.0019

摘要

以3個原生種及6個雜交種芭菲爾鞋蘭屬爲材料，取根尖經8羥基喹啉(8-Hydroxyquinoline)前處理及地衣紅(Orcein)染色後觀察其染色體長度及臂率比。P. insigne核型爲2n=26=26m、P. rothschildianum核型爲2n=26=22m + 4msm、變異種P. hirsutissimum var. esquirolei核型爲2n=30=26m + 4T、P. Goddodllar (P. primulinum × P. armeniacum)核型爲2n=30=22m + 2sm + 4msm + 2T、P. rothschildianum × P. micranthum 核型爲2n=28=26m + 2msm、P. Sunshine Glory 'B' (P. concolor albm × P. anthang-albm)核型爲2n=26=26m、P. kolopakingii 'Minoda' ×P. rothschildianum核型爲2n=28=14m + 6sm + 6msm + 2T、P. acmodontum × P. sukhakulii 核型爲2n=38=30m + 4msm + 4sm、P. liemianum × P. kolopakingii核型爲2n=32=30m + 2T。6個雜交種之後代體染色體數有2n=26、28、30、32及38等；依其親本染色體數，可分爲親本染色體數累加正常及偏離累加之型態；累加正常者有P. Sunshine Glory 'B'；另累加偏離者有P. Goddodllar 及P. liemianum × P. kolopakingii。又以P. rothschildianum 2n=26爲親本雜交P. kolopakingii 'Minoda'或與P. micranthum之後代亦屬於偏離累加之型態。

關鍵字

細胞分裂中期 ； 臂率比 ； 核型歧異 ； 染色體重排 ； 衛星體

並列摘要

The root tips of three Paphiopedilum species and six hybrids were used for this karyotype study. Active root tips were pretreated with 8-hydroxy-quinoline and then stained with orcein solution for chromosome measurements. P. insigne had 2n=26=26m karyotype, and P. rothschildianum had 2n=26=20m + 4msm + 2sm karyotype. The karyotype was 2n=30=26m + 4T for P. hirsutissimum var. esquirolei, 2n=30=22m + 2sm + 4msm + 2T for P. Goddodllar (P. primulinum × P. armeniacum), 2n=28=26m + 2msm for P. rothschildianum × P. micranthum, 2n=26=26m for P. Sunshine Glory 'B' (P. concolor albm × P. anthang-albm), 2n=28=14m + 6msm + 6sm + 2T for P. kolopakingii 'Minoda' × P. rothschildianum, 2n=38=30m + 4msm + 4sm for P. acmodontum × P. sukhakulii, and 2n=32=30m + 2T for P. liemianum × P. kolopakingii. The six hybrids were classified into additive and aberrant additive types according to the chromosome numbers of their parents. P. Sunshine Glory ‘B’ and P. acmodontum belonged to the additive type, while P. Goddodllar, P. liemianum × P. kolopakingii, and the hybrids between P. rothschildianum and P. kolopakingii 'Minoda' or P. rothschildianum and P. micranthum belonged to the aberrant additive types.

並列關鍵字

metaphase ； arm ratio ； karyotype diversification ； chromosome rearrangement ； satellite