Production of Induced Pluripotent Stem Cells by Reprogramming of Blood Cells

Sadia Zia ； Muhammad Zubair Yousaf ； Masroor Ellahi Babar ； Usman Ali Ashfaq

《International Journal of Animal and Veterinary Advances》 3卷3期 (2011/06) Pp. 193-195

並列摘要

Blood cells are the simple, efficient and economical source for the production of induced pluripotent cells. The discovery of induced pluripotent cells was not novel; it was pedestal on the scientific principals and technologies which have been developed over last six decades. These are nuclear transfer and the cloning of Animals, Pluripotent cell lines and fusion hybrids and Transcription Factors and lineage switching. The use of human embryonic stem cells in regenerative medicines was a breakthrough but make use of these cells arise ethical issues as they are obtained from human embryos. An alternative advancement using induced pluripotent stem cells, which mimics the embryonic stem cells has the significant gain that they replaced the embryonic stem cells. The pluripotent cells can be induced from terminally differentiated somatic cells by the Induction of only four defined factors including c-Myc, klf4, Oct4 and Sox2 which are enough to alter the fate of cell.

並列關鍵字

Fibroblasts ； C-Myc ； dedifferentiation ； iPSCs ； blood cells ； induced pluripotency ； proliferation ； reprogramming ； transcription factors