- 期刊
可量測細胞耗氧量之克拉克式氧氣感測晶片的研發
A Clark-type Oxygen Chip Capable of Measuring the Amount of Oxygen Consumption of Cells
摘要
近年來，透過微製程技術製作生物感測晶片已廣泛受重視，其中以細胞作爲辨識元件之微型生物感測晶片，可進一步發展爲細胞活性檢測平台。本研究開發一可量測細胞耗氧量之克拉克式氧氣感測晶片，以評估細胞數目與耗氧量之間的關係。研究中採用電沉積技術製作Ag/AgCl參考電極，可提供良好的電位穩定性，其使用壽命至少可維持40小時，電位漂移率僅0.35mV/h。此外，膠狀電解液的使用可防止外界的氧氣快速擴散到工作電極區，使此晶片的殘餘電流僅約6%，且晶片之靈敏度可提升至1.46nA/ppm，此晶片也成功測得不同細胞密度與耗氧量之間的關係。驗證此感測晶片具有微小化、便利與成本低之優點，未來可應用於快速檢測藥物或毒物對細胞之影響。
並列摘要
Recently, the bio-sensing chips fabricated by microfabrication techniques have widely attracted the attentions. Particularly, the miniature bio-sensing chips using cells as the recognition element can be developed to form a cell-activity evaluated platform. In this study, a Clark-type oxygen chip was developed for the measurement of the oxygen consumption of cells so as to estimate the relationship between the cell number and the amount of oxygen consumption. The Ag/AgCl was fabricated by the electrodeposition technique to be a pseudo-reference electrode; it presented a good stability and the lifetime of more than 40 h. The rate of potential shift was only 0.35 mV/h. In addition, the use of the alginate sol electrolyte can prevent the external oxygen molecules from fast diffusing to the sensitive area of working electrode. Moreover, the residual current can be decreased to 6% and the detecting sensitivity can be increased to 1.46 nA/ppm. The chip can successfully detect the relationship between the cell number and the amount of oxygen consumption. This sensing chip possessing the advantages of miniaturization, convenience, and low-cost can be applied in high throughput to detect the effect of drugs and toxicant on cells.