台灣地處亞熱帶地區加上四面環海,孕育豐富的海洋資源與種類繁多的農業產品,具有豐富的生物多樣性,非常有助於台灣在開發生質能源等相關原料來源,亦可解決目前多數能源包含生質能源仰賴於進口,而將造成受困於能源無法掌控,因此如何有效擴大利用現有資源為刻不容緩之事。本研究主要利用重綠葉馬尾藻(sargassum cristaefolium)以及香蕉收成後廢棄物(果梗)進行厭氧醱酵之可行性,並進而探討添加纖維水解酵素促進醱酵系統降解纖維的速率。第一階段以原料特性組成之C/N比例,對整個厭氧醱酵系統的影響性進行實驗。在批次的實驗結果,有機負荷在4gVS時,1:10的重綠葉馬尾藻與香蕉廢棄果梗的混合時,氣體產量為1,101.5 ± 0.2 ml/h濃度達到80%,明顯高於其他混合比例。第二階段為添加不同量之纖維水解酵素A-300(300 ml)、A-200(200 ml)、A-100(100 ml)及未添加之對照組進行,總產氣量結果以A-300及A-200達到1,982 ml/h和1,934 ml/h,明顯的高於對照組655 ml/h;在懸浮固形物中A-300及A-200的纖維降解情形亦優於其他組別,因此調整C/N比例及添加不同量之纖維水解酵素是有助於提升醱酵效率。
Taiwan is in the subtropical area surrounded by the sea, having a wealth of marine resources and a wide variety of agricultural products. The rich biological diversity is not only helpful in the development of biomass energy and other related sources of raw materials in Taiwan, but also solves the problem of uncontrollable energy (including biomass) that mostly imports from overseas. Thus, how to effectively expand and utilize the currently available resources is an imminent issue. This study mainly used sargassum cristaefolium and the post-harvest banana waste (banana pedicels) to study the feasibility of anaerobic fermentation, and thus to investigate the degradation rate of fermentation after adding cellulose. Initially, the impacts of varied C/N ratios on the whole anaerobic fermentation system were discussed and investigated. In batch experiments, when the organic load was 4gVS, mixed 1:10 sargassum cristaefolium and banana pedicels, the gas production was 1,101.5 ± 0.2 ml/h and the concentration 80%, which was significantly higher than the other mixture proportions. The second stage was to add various amounts of cellulose A-300 (300 ml), A-200 (200 ml), A-100 (100 ml) and compared with the non-added group (the control group); the total gas productions in A-300, A-200 were 1,982 ml/h and 1,934 ml/h respectively, which were also significantly higher than the non-added group of 655 ml/h; for the suspended solids in A-300 and A-200, the fiber degradation rates were better than the other groups. Thus, adjusting C/N ratios and adding different amounts of enzymes helped to improve the fermentation efficiency.