Stress and Group Housing Effects on the Survival of the Newly Proliferated Cell and Proliferative Neuroblast in the Dentate Gyrus

Li-Han Sun ； Lung Yu

《Adaptive Medicine》 10卷4期 (2018/12) Pp. 129-133

https://doi.org/10.4247/AM.2018.ABI214

摘要

A compound stressor regimen is known to rapidly induce reliable decreases in the newly proliferated cell and proliferative neuroblast in dentate gyrus (DG), while group housing may prevent such compound stressor- induced decreases. However, it remained elusive whether the short-lasting compound stressor regimen and the group housing may affect the survival of the newly proliferated cells and proliferative neuroblasts. We sought to assess whether the compound stressor regimen and group housing may affect the subsequent survival of newly proliferated cell or proliferative neuroblast using a 7-day post-stressor waiting period. An intraperitoneal injection of bromodeoxyuridine (BrdU, 100 mg/kg was given immediately before the beginning of the compound stressor regimen. Seven days following the conclusion of the compound stressor regimen, BrdU staining spot was used to indicate the survived, marked proliferated cell and BrdU co-staining with doublecortin (DCX) spot was used to reveal the survived, marked neuroblast. We found that mice receiving the compound stressors demonstrated the lowest number in survived marked proliferated cell and proliferative neuroblast among all groups. Moreover, the mice receiving the compound stressors and the group housing showed comparable number of marked proliferated cell and proliferative neuroblast as mice receiving no stressors. These results prompt us to suggest that the 1-hr compound stressors or the presence of cage-mates, once being conclud, do not seem to further affect the survival of the newly proliferated cell or proliferative neuroblast in DG.

關鍵字

dentate gyrus ； conspecific ； stress ； neurogenesis ； survival